Gene description for Skp1
Gene name S-phase kinase-associated protein 1
Gene symbol Skp1
Other names/aliases Skp1a
Species Rattus norvegicus
 Database cross references - Skp1
ExoCarta ExoCarta_287280
Entrez Gene 287280
UniProt Q6PEC4  
 Skp1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Skp1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 ISO
Biological Process
    SCF-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031146 ISS
    protein ubiquitination GO:0016567 ISO
    histone H2A monoubiquitination GO:0035518 ISO
Subcellular Localization
    SCF ubiquitin ligase complex GO:0019005 ISS
    Cul7-RING ubiquitin ligase complex GO:0031467 ISS
    PcG protein complex GO:0031519 ISO
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISS
 Experiment description of studies that identified Skp1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Skp1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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