Gene description for Shmt1
Gene name serine hydroxymethyltransferase 1 (soluble)
Gene symbol Shmt1
Other names/aliases LRRGT00032
Shmt
mShmt
Species Rattus norvegicus
 Database cross references - Shmt1
ExoCarta ExoCarta_287379
Vesiclepedia VP_287379
Entrez Gene 287379
 Shmt1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Shmt1
Molecular Function
    mRNA regulatory element binding translation repressor activity GO:0000900 ISO
    glycine hydroxymethyltransferase activity GO:0004372 IBA
    glycine hydroxymethyltransferase activity GO:0004372 IDA
    glycine hydroxymethyltransferase activity GO:0004372 ISO
    glycine hydroxymethyltransferase activity GO:0004372 TAS
    L-allo-threonine aldolase activity GO:0008732 IDA
    amino acid binding GO:0016597 IPI
    aldehyde-lyase activity GO:0016832 ISO
    pyridoxal phosphate binding GO:0030170 IBA
    pyridoxal phosphate binding GO:0030170 IDA
    pyridoxal phosphate binding GO:0030170 ISO
    pyridoxal phosphate binding GO:0030170 TAS
    small molecule binding GO:0036094 ISO
    identical protein binding GO:0042802 IPI
    identical protein binding GO:0042802 ISO
    protein homodimerization activity GO:0042803 ISO
    mRNA 5'-UTR binding GO:0048027 ISO
    serine binding GO:0070905 ISO
Biological Process
    dTMP biosynthetic process GO:0006231 ISO
    glycine metabolic process GO:0006544 IDA
    glycine metabolic process GO:0006544 ISO
    glycine metabolic process GO:0006544 TAS
    glycine biosynthetic process GO:0006545 IDA
    L-serine metabolic process GO:0006563 ISO
    L-serine metabolic process GO:0006563 TAS
    L-serine catabolic process GO:0006565 ISO
    purine nucleobase biosynthetic process GO:0009113 ISO
    negative regulation of translation GO:0017148 ISO
    glycine biosynthetic process from serine GO:0019264 IBA
    glycine biosynthetic process from serine GO:0019264 IDA
    tetrahydrofolate interconversion GO:0035999 IEA
    tetrahydrofolate interconversion GO:0035999 ISO
    tetrahydrofolate metabolic process GO:0046653 IBA
    tetrahydrofolate metabolic process GO:0046653 ISO
    folic acid metabolic process GO:0046655 ISO
    protein homotetramerization GO:0051289 ISO
    cellular response to tetrahydrofolate GO:1904482 ISO
    cellular response to leukemia inhibitory factor GO:1990830 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Shmt1 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Shmt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Gja1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Shmt1 is involved
No pathways found





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