Gene description for Ptrh2
Gene name peptidyl-tRNA hydrolase 2
Gene symbol Ptrh2
Other names/aliases RGD1306819
Species Rattus norvegicus
 Database cross references - Ptrh2
ExoCarta ExoCarta_287593
Vesiclepedia VP_287593
Entrez Gene 287593
 Ptrh2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Ptrh2
Molecular Function
    aminoacyl-tRNA hydrolase activity GO:0004045 IBA
    aminoacyl-tRNA hydrolase activity GO:0004045 ISO
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of gene expression GO:0010629 ISO
    positive regulation of anoikis GO:2000210 IBA
    positive regulation of anoikis GO:2000210 ISO
    negative regulation of anoikis GO:2000811 IBA
    negative regulation of anoikis GO:2000811 ISO
Subcellular Localization
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Ptrh2 in sEVs
1
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ptrh2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ptrh2 is involved
No pathways found





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