Gene description for Nit2
Gene name nitrilase family, member 2
Gene symbol Nit2
Other names/aliases RGD1310494
Species Rattus norvegicus
 Database cross references - Nit2
ExoCarta ExoCarta_288174
Vesiclepedia VP_288174
Entrez Gene 288174
UniProt Q497B0  
 Nit2 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nit2
Molecular Function
    hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides GO:0016811 IEA
    omega-amidase activity GO:0050152 IBA
    omega-amidase activity GO:0050152 IEA
    omega-amidase activity GO:0050152 ISO
    2-oxoglutaramate amidase activity GO:0106008 IEA
Biological Process
    oxaloacetate metabolic process GO:0006107 IBA
    oxaloacetate metabolic process GO:0006107 IEA
    oxaloacetate metabolic process GO:0006107 ISO
    asparagine metabolic process GO:0006528 IBA
    asparagine metabolic process GO:0006528 IEA
    asparagine metabolic process GO:0006528 ISO
    glutamine metabolic process GO:0006541 IBA
    glutamine metabolic process GO:0006541 IEA
    glutamine metabolic process GO:0006541 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    mitochondrion GO:0005739 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Nit2 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nit2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nit2 is involved
PathwayEvidenceSource
Immune System IEA Reactome
Innate Immune System IEA Reactome
Neutrophil degranulation IEA Reactome





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