Gene description for Nit2
Gene name nitrilase family, member 2
Gene symbol Nit2
Other names/aliases RGD1310494
Species Rattus norvegicus
 Database cross references - Nit2
ExoCarta ExoCarta_288174
Entrez Gene 288174
UniProt Q497B0  
 Nit2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nit2
Molecular Function
    omega-amidase activity GO:0050152 ISO
Biological Process
    glutamine metabolic process GO:0006541 ISO
    oxaloacetate metabolic process GO:0006107 ISO
    asparagine metabolic process GO:0006528 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 ISO
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Nit2 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nit2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nit2 is involved
No pathways found





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