Gene description for Hsph1
Gene name heat shock 105/110 protein 1
Gene symbol Hsph1
Other names/aliases Hsp105
Species Rattus norvegicus
 Database cross references - Hsph1
ExoCarta ExoCarta_288444
Entrez Gene 288444
UniProt Q66HA8  
 Hsph1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hsph1
Molecular Function
    ATP binding GO:0005524 IEA
    alpha-tubulin binding GO:0043014 ISO
Biological Process
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    negative regulation of establishment of protein localization to mitochondrion GO:1903748 ISO
    negative regulation of p38MAPK cascade GO:1903753 ISO
    negative regulation of apoptotic signaling pathway GO:2001234 ISO
    chaperone mediated protein folding requiring cofactor GO:0051085 ISO
    positive regulation of protein tyrosine kinase activity GO:0061098 ISO
    negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:1903751 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 ISO
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Hsph1 in exosomes
1
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hsph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hsph1 is involved
PathwayEvidenceSource
Regulation of HSF1-mediated heat shock response IEA Reactome
Scavenging by Class F Receptors IEA Reactome





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