Gene description for Hsph1
Gene name heat shock 105/110 protein 1
Gene symbol Hsph1
Other names/aliases Hsp105
Species Rattus norvegicus
 Database cross references - Hsph1
ExoCarta ExoCarta_288444
Vesiclepedia VP_288444
Entrez Gene 288444
UniProt Q66HA8  
 Hsph1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hsph1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    adenyl-nucleotide exchange factor activity GO:0000774 ISO
    adenyl-nucleotide exchange factor activity GO:0000774 ISS
    ATP binding GO:0005524 IEA
    alpha-tubulin binding GO:0043014 ISO
    ATP-dependent protein folding chaperone GO:0140662 IEA
Biological Process
    protein folding GO:0006457 IBA
    protein folding GO:0006457 IEA
    chaperone cofactor-dependent protein refolding GO:0051085 ISO
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 ISO
    microtubule GO:0005874 ISO
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Hsph1 in sEVs
1
Experiment ID 94
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hsph1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Grb7  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



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