Gene description for Mcm7
Gene name minichromosome maintenance complex component 7
Gene symbol Mcm7
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Mcm7
ExoCarta ExoCarta_288532
Entrez Gene 288532
 Mcm7 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Mcm7
Molecular Function
    DNA helicase activity GO:0003678 ISO
    DNA-dependent ATPase activity GO:0008094 TAS
    DNA binding GO:0003677 TAS
    ATP binding GO:0005524 TAS
    ATP-dependent DNA helicase activity GO:0004003 ISO
    single-stranded DNA binding GO:0003697 ISO
Biological Process
    DNA replication GO:0006260 TAS
    cell proliferation GO:0008283 TAS
    cellular response to DNA damage stimulus GO:0006974 ISO
    cellular response to xenobiotic stimulus GO:0071466 ISO
    response to drug GO:0042493 IEP
    DNA unwinding involved in DNA replication GO:0006268 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IEP
    cellular response to organic substance GO:0071310 IEP
    DNA replication initiation GO:0006270 IEA
    regulation of phosphorylation GO:0042325 ISO
    regulation of transcription, DNA-templated GO:0006355 TAS
Subcellular Localization
    cytoplasm GO:0005737 ISO
    membrane GO:0016020 ISO
    cytosol GO:0005829 IDA
    nucleus GO:0005634 ISO
    MCM complex GO:0042555 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Mcm7 in exosomes
1
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Mcm7
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Mcm7 is involved
No pathways found





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