Gene description for Mcm7
Gene name minichromosome maintenance complex component 7
Gene symbol Mcm7
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Mcm7
ExoCarta ExoCarta_288532
Vesiclepedia VP_288532
Entrez Gene 288532
 Mcm7 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Mcm7
Molecular Function
    DNA binding GO:0003677 TAS
    DNA helicase activity GO:0003678 ISO
    single-stranded DNA binding GO:0003697 ISO
    single-stranded DNA binding GO:0003697 ISO
    ATP binding GO:0005524 TAS
    ATP-dependent activity, acting on DNA GO:0008094 TAS
    hydrolase activity GO:0016787 IEA
    ATP hydrolysis activity GO:0016887 IEA
Biological Process
    DNA replication GO:0006260 TAS
    DNA unwinding involved in DNA replication GO:0006268 ISO
    DNA unwinding involved in DNA replication GO:0006268 ISO
    regulation of DNA-templated transcription GO:0006355 TAS
    DNA damage response GO:0006974 ISO
    cell population proliferation GO:0008283 ISO
    response to xenobiotic stimulus GO:0009410 IEP
    regulation of phosphorylation GO:0042325 ISO
    cellular response to epidermal growth factor stimulus GO:0071364 IEP
    cellular response to xenobiotic stimulus GO:0071466 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleolus GO:0005730 ISO
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    MCM complex GO:0042555 ISO
    CMG complex GO:0071162 ISO
 Experiment description of studies that identified Mcm7 in sEVs
1
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [MALDI TOF]
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Mcm7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Sumo3  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Mcm7 is involved
No pathways found





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