Gene description for Naca
Gene name nascent polypeptide-associated complex alpha subunit
Gene symbol Naca
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Naca
ExoCarta ExoCarta_288770
Vesiclepedia VP_288770
Entrez Gene 288770
 Naca identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Naca
Molecular Function
    transcription coactivator activity GO:0003713 ISO
    TBP-class protein binding GO:0017025 ISO
    unfolded protein binding GO:0051082 IBA
Biological Process
    protein targeting to membrane GO:0006612 IBA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    myoblast migration GO:0051451 ISO
    negative regulation of protein localization to endoplasmic reticulum GO:1905551 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 ISO
    nascent polypeptide-associated complex GO:0005854 ISO
 Experiment description of studies that identified Naca in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Naca
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Naca is involved
No pathways found





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