ExoCarta: Gene summary

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Gene description for Naca
Gene name	nascent polypeptide-associated complex alpha subunit
Gene symbol	Naca
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Naca
ExoCarta	ExoCarta_288770
Entrez Gene	288770
Naca identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Naca

Molecular Function
	TBP-class protein binding	GO:0017025	ISO
	transcription coactivator activity	GO:0003713	ISO
Biological Process
	positive regulation of transcription from RNA polymerase II promoter involved in heart development	GO:1901228	ISO
	heart trabecula morphogenesis	GO:0061384	ISO
	negative regulation of transcription from RNA polymerase II promoter involved in heart development	GO:1901227	ISO
	regulation of nucleic acid-templated transcription	GO:1903506	ISO
	negative regulation of striated muscle cell apoptotic process	GO:0010664	ISO
	cardiac ventricle development	GO:0003231	ISO
	positive regulation of skeletal muscle tissue growth	GO:0048633	ISO
	regulation of skeletal muscle fiber development	GO:0048742	ISO
	positive regulation of cell proliferation involved in heart morphogenesis	GO:2000138	ISO
	skeletal muscle tissue regeneration	GO:0043403	ISO
	myoblast migration	GO:0051451	ISO
Subcellular Localization
	cytoplasm	GO:0005737	ISO
	nucleus	GO:0005634	ISO
	extracellular exosome	GO:0070062	ISO

Experiment description of studies that identified Naca in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D4 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting