ExoCarta: Gene summary

Credits | Contact

Gene description for Mri1
Gene name	methylthioribose-1-phosphate isomerase 1
Gene symbol	Mri1
Other names/aliases	RGD1307789
Species	Rattus norvegicus
Database cross references - Mri1
ExoCarta	ExoCarta_288912
Entrez Gene	288912
UniProt	Q5HZE4
Mri1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Mri1

Molecular Function
	identical protein binding	GO:0042802	ISO
	S-methyl-5-thioribose-1-phosphate isomerase activity	GO:0046523	IBA
Biological Process
	L-methionine biosynthetic process from S-adenosylmethionine	GO:0019284	IEA
	L-methionine biosynthetic process from methylthioadenosine	GO:0019509	IEA
Subcellular Localization
	nucleolus	GO:0005730	ISO
	cytoplasm	GO:0005737	ISO

Experiment description of studies that identified Mri1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting