ExoCarta: Gene summary

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Gene description for Mri1
Gene name	methylthioribose-1-phosphate isomerase 1
Gene symbol	Mri1
Other names/aliases	RGD1307789
Species	Rattus norvegicus
Database cross references - Mri1
ExoCarta	ExoCarta_288912
Vesiclepedia	VP_288912
Entrez Gene	288912
UniProt	Q5HZE4
Mri1 identified in sEVs derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Mri1

Molecular Function
	identical protein binding	GO:0042802	ISO
	S-methyl-5-thioribose-1-phosphate isomerase activity	GO:0046523	IBA
Biological Process
	L-methionine salvage from methylthioadenosine	GO:0019509	IBA
	L-methionine salvage from methylthioadenosine	GO:0019509	IEA
Subcellular Localization
	fibrillar center	GO:0001650	IEA
	fibrillar center	GO:0001650	ISO
	nucleoplasm	GO:0005654	IEA
	nucleoplasm	GO:0005654	ISO
	cytosol	GO:0005829	IEA
	cytosol	GO:0005829	ISO

Experiment description of studies that identified Mri1 in sEVs

Experiment ID

MISEV standards
✔ EM	Biophysical techniques
✔ TSG101\|HSP90\|HSC70\|MHCI	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting