Gene description for Hmcn1
Gene name hemicentin 1
Gene symbol Hmcn1
Other names/aliases RGD1564772
Species Rattus norvegicus
 Database cross references - Hmcn1
ExoCarta ExoCarta_289094
Entrez Gene 289094
 Hmcn1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Hmcn1
Molecular Function
    calcium ion binding GO:0005509 IEA
Subcellular Localization
    cell cortex GO:0005938 ISO
    basement membrane GO:0005604 ISO
    extracellular exosome GO:0070062 ISO
    cell junction GO:0030054 ISO
    extracellular region GO:0005576 ISO
 Experiment description of studies that identified Hmcn1 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Hmcn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hmcn1 is involved
No pathways found





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