Gene description for IGKV3-20
Gene name immunoglobulin kappa variable 3-20
Gene symbol IGKV3-20
Other names/aliases 13K18
A27
IGKV320
Species Homo sapiens
 Database cross references - IGKV3-20
ExoCarta ExoCarta_28912
Entrez Gene 28912
HGNC 5817
 IGKV3-20 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Saliva 19199708    
 Gene ontology annotations for IGKV3-20
Molecular Function
    immunoglobulin receptor binding GO:0034987 IDA
    antigen binding GO:0003823 NAS
Biological Process
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    receptor-mediated endocytosis GO:0006898 TAS
    complement activation GO:0006956 TAS
    immune response GO:0006955 NAS
    antibacterial humoral response GO:0019731 IDA
    glomerular filtration GO:0003094 IMP
    complement activation, classical pathway GO:0006958 TAS
    innate immune response GO:0045087 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    regulation of immune response GO:0050776 TAS
Subcellular Localization
    monomeric IgA immunoglobulin complex GO:0071748 IDA
    secretory IgA immunoglobulin complex GO:0071751 IDA
    extracellular space GO:0005615 IDA
    blood microparticle GO:0072562 IDA
    plasma membrane GO:0005886 TAS
    pentameric IgM immunoglobulin complex GO:0071756 IDA
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
 Experiment description of studies that identified IGKV3-20 in exosomes
1
Experiment ID 21
ISEV standards
EM|IEM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for IGKV3-20
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which IGKV3-20 is involved
No pathways found





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