Gene description for Dhx15
Gene name DEAH (Asp-Glu-Ala-His) box helicase 15
Gene symbol Dhx15
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dhx15
ExoCarta ExoCarta_289693
Vesiclepedia VP_289693
Entrez Gene 289693
 Dhx15 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dhx15
Molecular Function
    RNA binding GO:0003723 IBA
    RNA binding GO:0003723 ISO
    RNA helicase activity GO:0003724 ISO
    double-stranded RNA binding GO:0003725 ISO
    helicase activity GO:0004386 IBA
    ATP binding GO:0005524 IEA
    hydrolase activity GO:0016787 IEA
Biological Process
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    response to toxic substance GO:0009636 IEP
    defense response to bacterium GO:0042742 ISO
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 ISO
    response to alkaloid GO:0043279 IEP
    defense response to virus GO:0051607 ISO
    antiviral innate immune response GO:0140374 ISO
Subcellular Localization
    spliceosomal complex GO:0005681 IBA
    U12-type spliceosomal complex GO:0005689 ISO
    membrane GO:0016020 IEA
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    U2-type post-mRNA release spliceosomal complex GO:0071008 ISO
 Experiment description of studies that identified Dhx15 in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dhx15
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dhx15 is involved
No pathways found





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