ExoCarta: Gene summary

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Gene description for Ewsr1
Gene name	EWS RNA-binding protein 1
Gene symbol	Ewsr1
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Ewsr1
ExoCarta	ExoCarta_289752
Entrez Gene	289752
Ewsr1 identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Ewsr1

Molecular Function
	identical protein binding	GO:0042802	ISO
	nucleotide binding	GO:0000166	IEA
	zinc ion binding	GO:0008270	IEA
	poly(A) RNA binding	GO:0044822	ISO
	nucleic acid binding	GO:0003676	IEA
	molecular_function	GO:0003674	ND
Biological Process
	biological_process	GO:0008150	ND
Subcellular Localization
	cytoplasm	GO:0005737	IDA
	Cajal body	GO:0015030	IDA
	nucleolus	GO:0005730	ISO
	nucleoplasm	GO:0005654	ISO
	nucleus	GO:0005634	ISO

Experiment description of studies that identified Ewsr1 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting