Gene description for Pnp
Gene name purine nucleoside phosphorylase
Gene symbol Pnp
Other names/aliases Np
Species Rattus norvegicus
 Database cross references - Pnp
ExoCarta ExoCarta_290029
Entrez Gene 290029
UniProt P85973  
 Pnp identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
 Gene ontology annotations for Pnp
Molecular Function
    purine-nucleoside phosphorylase activity GO:0004731 ISO
    phosphate ion binding GO:0042301 ISO
    drug binding GO:0008144 ISO
    nucleoside binding GO:0001882 ISO
    purine nucleobase binding GO:0002060 ISO
Biological Process
    positive regulation of T cell proliferation GO:0042102 ISO
    immune response GO:0006955 ISO
    nicotinamide riboside catabolic process GO:0006738 ISO
    purine-containing compound salvage GO:0043101 TAS
    nucleoside metabolic process GO:0009116 IEA
    urate biosynthetic process GO:0034418 ISO
    purine nucleoside metabolic process GO:0042278 IDA
    interleukin-2 secretion GO:0070970 ISO
    NAD biosynthesis via nicotinamide riboside salvage pathway GO:0034356 ISO
    nucleobase-containing compound metabolic process GO:0006139 ISO
    inosine catabolic process GO:0006148 ISO
    positive regulation of alpha-beta T cell differentiation GO:0046638 ISO
    response to drug GO:0042493 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    extracellular region GO:0005576 IDA
    nucleus GO:0005634 IDA
    intracellular GO:0005622 ISO
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified Pnp in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for Pnp
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Pnp is involved
No pathways found





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