Gene description for Usp4
Gene name ubiquitin specific peptidase 4 (proto-oncogene)
Gene symbol Usp4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Usp4
ExoCarta ExoCarta_290864
Vesiclepedia VP_290864
Entrez Gene 290864
UniProt B2GUZ1  
 Usp4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Usp4
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IEA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    cysteine-type deubiquitinase activity GO:0004843 ISS
    adenosine receptor binding GO:0031685 ISO
    identical protein binding GO:0042802 ISO
    metal ion binding GO:0046872 IEA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    proteolysis GO:0006508 IEA
    protein deubiquitination GO:0016579 IEA
    protein deubiquitination GO:0016579 ISO
    protein deubiquitination GO:0016579 ISS
    negative regulation of protein ubiquitination GO:0031397 ISO
    negative regulation of protein ubiquitination GO:0031397 ISS
    regulation of protein stability GO:0031647 ISO
    regulation of protein stability GO:0031647 ISS
    protein localization to cell surface GO:0034394 ISO
    protein localization to cell surface GO:0034394 ISS
    positive regulation of TORC1 signaling GO:1904263 ISO
    positive regulation of TORC1 signaling GO:1904263 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IEA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Usp4 in sEVs
1
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Usp4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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