ExoCarta: Gene summary

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Gene description for Serpinb1a
Gene name	serine (or cysteine) proteinase inhibitor, clade B, member 1a
Gene symbol	Serpinb1a
Other names/aliases	-
Species	Rattus norvegicus
Database cross references - Serpinb1a
ExoCarta	ExoCarta_291091
Entrez Gene	291091
UniProt	Q4G075
Serpinb1a identified in exosomes derived from the following tissue/cell type
Reticulocytes	21828046
Reticulocytes	21828046

Gene ontology annotations for Serpinb1a

Molecular Function
	serine-type endopeptidase inhibitor activity	GO:0004867	IBA
Biological Process
	negative regulation of endopeptidase activity	GO:0010951	IBA
	regulation of protein catabolic process	GO:0042176	ISO
Subcellular Localization
	membrane	GO:0016020	ISO
	cytoplasm	GO:0005737	IEA
	extracellular space	GO:0005615	ISO
	extracellular exosome	GO:0070062	ISO

Experiment description of studies that identified Serpinb1a in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✔ TSG101\|HSP90\|HSC70	EV Cytosolic markers
✔ MHCI	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [Orbitrap]

PubMed ID

21828046

Organism

Rattus norvegicus

Experiment description

The proteo-lipidic composition of exosomes changes during reticulocyte maturation.

Authors

Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.

Journal name

JBC

Publication year

2011

Sample

Reticulocytes

Sample name

Reticulocytes - D2 Rep 2

Isolation/purification methods

Differential centrifugation
Ultracentrifugation
Sucrose density gradient

Flotation density

1.16-1.21 g/mL

Molecules identified in the study

Protein
Lipids

Methods used in the study

Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting