Gene description for Dctn1
Gene name dynactin 1
Gene symbol Dctn1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dctn1
ExoCarta ExoCarta_29167
Entrez Gene 29167
UniProt P28023  
 Dctn1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Dctn1
Molecular Function
    dynein binding GO:0045502 IEA
    microtubule motor activity GO:0003777 TAS
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 ISO
    positive regulation of intracellular protein transport GO:0090316 ISS
    microtubule-based process GO:0007017 TAS
    microtubule-based transport GO:0010970 IEA
    metabolic process GO:0008152 TAS
    cytoplasmic microtubule organization GO:0031122 ISS
    melanosome transport GO:0032402 ISO
Subcellular Localization
    cell leading edge GO:0031252 ISO
    centrosome GO:0005813 ISS
    cell GO:0005623 ISO
    microtubule GO:0005874 ISO
    protein complex GO:0043234 ISO
    kinetochore GO:0000776 ISO
    spindle pole GO:0000922 ISO
    membrane GO:0016020 ISO
    cytoplasmic dynein complex GO:0005868 IDA
    dynactin complex GO:0005869 IEA
    retromer complex GO:0030904 ISO
 Experiment description of studies that identified Dctn1 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dctn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dctn1 is involved
No pathways found





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