Gene description for Dctn1
Gene name dynactin 1
Gene symbol Dctn1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dctn1
ExoCarta ExoCarta_29167
Vesiclepedia VP_29167
Entrez Gene 29167
UniProt P28023  
 Dctn1 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Dctn1
Molecular Function
    microtubule binding GO:0008017 IDA
    microtubule binding GO:0008017 IEA
    microtubule binding GO:0008017 ISO
    tubulin binding GO:0015631 ISO
    tubulin binding GO:0015631 ISS
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
    identical protein binding GO:0042802 IPI
    molecular adaptor activity GO:0060090 EXP
Biological Process
    establishment of mitotic spindle orientation GO:0000132 IBA
    establishment of mitotic spindle orientation GO:0000132 IEA
    establishment of mitotic spindle orientation GO:0000132 ISO
    establishment of mitotic spindle orientation GO:0000132 ISS
    nuclear migration GO:0007097 IBA
    neuromuscular junction development GO:0007528 IEA
    neuromuscular junction development GO:0007528 ISO
    biological_process GO:0008150 ND
    centriole-centriole cohesion GO:0010457 IEA
    centriole-centriole cohesion GO:0010457 ISO
    centriole-centriole cohesion GO:0010457 ISS
    ventral spinal cord development GO:0021517 IEA
    ventral spinal cord development GO:0021517 ISO
    positive regulation of microtubule polymerization GO:0031116 ISO
    positive regulation of microtubule polymerization GO:0031116 ISS
    cytoplasmic microtubule organization GO:0031122 ISS
    melanosome transport GO:0032402 IEA
    melanosome transport GO:0032402 ISO
    microtubule anchoring at centrosome GO:0034454 IEA
    microtubule anchoring at centrosome GO:0034454 ISO
    microtubule anchoring at centrosome GO:0034454 ISS
    retrograde transport, endosome to Golgi GO:0042147 IEA
    retrograde transport, endosome to Golgi GO:0042147 ISO
    neuromuscular process GO:0050905 IEA
    neuromuscular process GO:0050905 ISO
    nuclear membrane disassembly GO:0051081 IEA
    nuclear membrane disassembly GO:0051081 ISO
    nuclear membrane disassembly GO:0051081 ISS
    cell division GO:0051301 IEA
    regulation of mitotic spindle organization GO:0060236 IEA
    regulation of mitotic spindle organization GO:0060236 ISO
    regulation of mitotic spindle organization GO:0060236 ISS
    motor behavior GO:0061744 IEA
    motor behavior GO:0061744 ISO
    neuron cellular homeostasis GO:0070050 IEA
    neuron cellular homeostasis GO:0070050 ISO
    positive regulation of microtubule nucleation GO:0090063 IEA
    positive regulation of microtubule nucleation GO:0090063 ISO
    positive regulation of microtubule nucleation GO:0090063 ISS
    positive regulation of intracellular protein transport GO:0090316 ISS
    axonal transport GO:0098930 ISO
    positive regulation of neuromuscular junction development GO:1904398 IEA
    positive regulation of neuromuscular junction development GO:1904398 ISO
    non-motile cilium assembly GO:1905515 IEA
    non-motile cilium assembly GO:1905515 ISO
    non-motile cilium assembly GO:1905515 ISS
    neuron projection maintenance GO:1990535 IEA
    neuron projection maintenance GO:1990535 ISO
Subcellular Localization
    kinetochore GO:0000776 IBA
    kinetochore GO:0000776 IEA
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    spindle pole GO:0000922 IBA
    spindle pole GO:0000922 IEA
    spindle pole GO:0000922 ISO
    nuclear envelope GO:0005635 IEA
    nuclear envelope GO:0005635 ISO
    nuclear envelope GO:0005635 ISS
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    centrosome GO:0005813 ISS
    centriole GO:0005814 IEA
    centriole GO:0005814 ISO
    centriole GO:0005814 ISS
    spindle GO:0005819 ISO
    spindle GO:0005819 ISS
    cytosol GO:0005829 IEA
    cytoplasmic dynein complex GO:0005868 IDA
    microtubule GO:0005874 IEA
    microtubule GO:0005874 ISO
    microtubule GO:0005874 ISS
    microtubule associated complex GO:0005875 IBA
    microtubule associated complex GO:0005875 ISO
    cell cortex GO:0005938 IEA
    cell cortex GO:0005938 ISO
    cell cortex GO:0005938 ISS
    microtubule cytoskeleton GO:0015630 ISO
    dynein complex GO:0030286 IEA
    axon GO:0030424 IBA
    retromer complex GO:0030904 IEA
    retromer complex GO:0030904 ISO
    cell leading edge GO:0031252 IEA
    cell leading edge GO:0031252 ISO
    protein-containing complex GO:0032991 ISO
    microtubule plus-end GO:0035371 IEA
    microtubule plus-end GO:0035371 ISO
    microtubule plus-end GO:0035371 ISS
    ciliary basal body GO:0036064 IEA
    ciliary basal body GO:0036064 ISO
    neuron projection GO:0043005 IEA
    neuron projection GO:0043005 ISO
    neuronal cell body GO:0043025 IEA
    neuronal cell body GO:0043025 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
    centriolar subdistal appendage GO:0120103 IEA
    centriolar subdistal appendage GO:0120103 ISO
 Experiment description of studies that identified Dctn1 in sEVs
1
Experiment ID 95
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dctn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ABCG1  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Dctn1 is involved
PathwayEvidenceSource
Adaptive Immune System IEA Reactome
Anchoring of the basal body to the plasma membrane IEA Reactome
Asparagine N-linked glycosylation IEA Reactome
AURKA Activation by TPX2 IEA Reactome
Cell Cycle IEA Reactome
Cell Cycle, Mitotic IEA Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress IEA Reactome
Centrosome maturation IEA Reactome
Cilium Assembly IEA Reactome
COPI-independent Golgi-to-ER retrograde traffic IEA Reactome
COPI-mediated anterograde transport IEA Reactome
ER to Golgi Anterograde Transport IEA Reactome
G2/M Transition IEA Reactome
Golgi-to-ER retrograde transport IEA Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand IEA Reactome
Immune System IEA Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic IEA Reactome
Loss of Nlp from mitotic centrosomes IEA Reactome
Loss of proteins required for interphase microtubule organization from the centrosome IEA Reactome
M Phase IEA Reactome
Membrane Trafficking IEA Reactome
Metabolism of proteins IEA Reactome
MHC class II antigen presentation IEA Reactome
Mitotic G2-G2/M phases IEA Reactome
Mitotic Prometaphase IEA Reactome
Organelle biogenesis and maintenance IEA Reactome
Post-translational protein modification IEA Reactome
Recruitment of mitotic centrosome proteins and complexes IEA Reactome
Recruitment of NuMA to mitotic centrosomes IEA Reactome
Regulation of PLK1 Activity at G2/M Transition IEA Reactome
Transport to the Golgi and subsequent modification IEA Reactome
Vesicle-mediated transport IEA Reactome





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