Gene description for Usp14
Gene name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Gene symbol Usp14
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Usp14
ExoCarta ExoCarta_291796
Entrez Gene 291796
 Usp14 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Usp14
Molecular Function
    ubiquitin-specific protease activity GO:0004843 ISO
    endopeptidase inhibitor activity GO:0004866 ISO
    proteasome binding GO:0070628 ISO
    cysteine-type endopeptidase activity GO:0004197 IBA
Biological Process
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    synaptic transmission GO:0007268 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein deubiquitination GO:0016579 ISO
    negative regulation of endopeptidase activity GO:0010951 ISO
    regulation of chemotaxis GO:0050920 ISO
    negative regulation of ER-associated ubiquitin-dependent protein catabolic process GO:1903070 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    nucleus GO:0005634 IBA
    cytoplasmic membrane-bounded vesicle GO:0016023 ISO
    cytoplasm GO:0005737 ISO
    cell surface GO:0009986 ISO
    synapse GO:0045202 ISO
 Experiment description of studies that identified Usp14 in exosomes
Experiment ID 95
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Usp14
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Usp14 is involved
No pathways found

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here