Gene description for Usp14
Gene name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Gene symbol Usp14
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Usp14
ExoCarta ExoCarta_291796
Vesiclepedia VP_291796
Entrez Gene 291796
 Usp14 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Usp14
Molecular Function
    cysteine-type deubiquitinase activity GO:0004843 IBA
    cysteine-type deubiquitinase activity GO:0004843 ISO
    endopeptidase inhibitor activity GO:0004866 ISO
    K63-linked deubiquitinase activity GO:0061578 ISO
    proteasome binding GO:0070628 IBA
    proteasome binding GO:0070628 ISO
    deubiquitinase activity GO:0101005 ISO
Biological Process
    chemical synaptic transmission GO:0007268 ISO
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    regulation of chemotaxis GO:0050920 ISO
    regulation of proteasomal protein catabolic process GO:0061136 ISO
    protein K48-linked deubiquitination GO:0071108 ISO
    negative regulation of ERAD pathway GO:1904293 IBA
    negative regulation of ERAD pathway GO:1904293 ISO
    negative regulation of ubiquitin-dependent protein catabolic process GO:2000059 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    cell surface GO:0009986 ISO
    cytoplasmic vesicle GO:0031410 ISO
    synapse GO:0045202 ISO
    glutamatergic synapse GO:0098978 ISO
    presynaptic cytosol GO:0099523 ISO
 Experiment description of studies that identified Usp14 in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Usp14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rad23b  
Affinity Capture-MS Rattus norvegicus
2 GABBR1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which Usp14 is involved
No pathways found





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