Gene description for Cd63
Gene name Cd63 molecule
Gene symbol Cd63
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Cd63
ExoCarta ExoCarta_29186
Vesiclepedia VP_29186
Entrez Gene 29186
UniProt P28648  
 Cd63 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 19367702    
Hippocampal neuronal cells 34654817    
Hippocampal neuronal cells 34654817    
Neural stem cells 31848325    
Neural stem cells 31848325    
Schwann cells 36717543    
Serum 33159193    
 Gene ontology annotations for Cd63
Molecular Function
    protein binding GO:0005515 IPI
    protein-containing complex binding GO:0044877 IDA
Biological Process
    positive regulation of receptor internalization GO:0002092 IEA
    positive regulation of receptor internalization GO:0002092 ISO
    positive regulation of receptor internalization GO:0002092 ISS
    cell-matrix adhesion GO:0007160 IEA
    cell-matrix adhesion GO:0007160 ISO
    cell-matrix adhesion GO:0007160 ISS
    negative regulation of epithelial cell migration GO:0010633 IMP
    protein transport GO:0015031 IEA
    cell migration GO:0016477 IEA
    cell migration GO:0016477 ISO
    cell migration GO:0016477 ISS
    epithelial cell differentiation GO:0030855 IMP
    endosome to melanosome transport GO:0035646 IEA
    endosome to melanosome transport GO:0035646 ISO
    endosome to melanosome transport GO:0035646 ISS
    pigmentation GO:0043473 ISO
    positive regulation of cell adhesion GO:0045785 IMP
    positive regulation of endocytosis GO:0045807 IMP
    pigment granule maturation GO:0048757 ISO
    pigment granule maturation GO:0048757 ISS
    pigment cell differentiation GO:0050931 ISO
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 IBA
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 IEA
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 ISO
    regulation of vascular endothelial growth factor signaling pathway GO:1900746 ISS
    regulation of potassium ion transmembrane transport GO:1901379 IEA
    regulation of potassium ion transmembrane transport GO:1901379 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 IEA
    positive regulation of integrin-mediated signaling pathway GO:2001046 ISO
    positive regulation of integrin-mediated signaling pathway GO:2001046 ISS
Subcellular Localization
    extracellular region GO:0005576 IEA
    extracellular space GO:0005615 ISO
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    lysosome GO:0005764 ISO
    lysosomal membrane GO:0005765 IEA
    lysosomal membrane GO:0005765 ISO
    lysosomal membrane GO:0005765 ISS
    late endosome GO:0005770 ISO
    multivesicular body GO:0005771 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    cell surface GO:0009986 IEA
    cell surface GO:0009986 ISO
    endosome membrane GO:0010008 ISO
    late endosome membrane GO:0031902 IEA
    late endosome membrane GO:0031902 ISO
    late endosome membrane GO:0031902 ISS
    endosome lumen GO:0031904 IEA
    endosome lumen GO:0031904 ISO
    multivesicular body membrane GO:0032585 IEA
    multivesicular body membrane GO:0032585 ISO
    multivesicular body membrane GO:0032585 ISS
    protein-containing complex GO:0032991 IDA
    melanosome GO:0042470 IEA
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 IDA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    multivesicular body, internal vesicle GO:0097487 IEA
    multivesicular body, internal vesicle GO:0097487 ISO
    multivesicular body, internal vesicle GO:0097487 ISS
 Experiment description of studies that identified Cd63 in sEVs
1
Experiment ID 225
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
7
Experiment ID 1160
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
8
Experiment ID 1161
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
9
Experiment ID 1217
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 31848325    
Organism Rattus norvegicus
Experiment description Insulin-like growth factor-1 enchances neuroprotective effects of neural stem cell exosomes after spinal cord injury via an miR-219a-2-3p/YY1 mechanism
Authors "Ma K, Xu H, Zhang J, Zhao F, Liang H, Sun H, Li P, Zhang S, Wang R, Chen X."
Journal name Aging
Publication year 2019
Sample Neural stem cells
Sample name Cerebral cortex-derived NSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
10
Experiment ID 1218
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 31848325    
Organism Rattus norvegicus
Experiment description Insulin-like growth factor-1 enchances neuroprotective effects of neural stem cell exosomes after spinal cord injury via an miR-219a-2-3p/YY1 mechanism
Authors "Ma K, Xu H, Zhang J, Zhao F, Liang H, Sun H, Li P, Zhang S, Wang R, Chen X."
Journal name Aging
Publication year 2019
Sample Neural stem cells
Sample name Cerebral cortex-derived NSCs
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
RNA sequencing
qRT-PCR
11
Experiment ID 678
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Western blotting
PubMed ID 36717543    
Organism Rattus norvegicus
Experiment description Schwann cell-derived exosomes containing MFG-E8 modify macrophage/microglial polarization for attenuating inflammation via the SOCS3/STAT3 pathway after spinal cord injury
Authors "Ren J, Zhu B, Gu G, Zhang W, Li J, Wang H, Wang M, Song X, Wei Z, Feng S."
Journal name Cell Death Dis
Publication year 2023
Sample Schwann cells
Sample name Sciatic nerve-derived schwann cells
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 323
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 33159193    
Organism Rattus norvegicus
Experiment description Treatment of infarcted heart tissue via the capture and local delivery of circulating exosomes through antibody-conjugated magnetic nanoparticles
Authors "Liu S, Chen X, Bao L, Liu T, Yuan P, Yang X, Qiu X, Gooding JJ, Bai Y, Xiao J, Pu F, Jin Y. "
Journal name Nat Biomed Eng
Publication year 2020
Sample Serum
Sample name Serum - Sprague Dawley rats
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
 Protein-protein interactions for Cd63
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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