Gene description for Sf3b3
Gene name splicing factor 3b, subunit 3
Gene symbol Sf3b3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Sf3b3
ExoCarta ExoCarta_292019
Entrez Gene 292019
 Sf3b3 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Sf3b3
Molecular Function
    RNA binding GO:0003723 IBA
Biological Process
    negative regulation of protein catabolic process GO:0042177 ISO
    mRNA splicing, via spliceosome GO:0000398 IBA
Subcellular Localization
    U12-type spliceosomal complex GO:0005689 ISO
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Sf3b3 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sf3b3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Sf3b3 is involved
No pathways found





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