Gene description for Tubb4a
Gene name tubulin, beta 4A class IVa
Gene symbol Tubb4a
Other names/aliases Tubb4
Species Rattus norvegicus
 Database cross references - Tubb4a
ExoCarta ExoCarta_29213
Entrez Gene 29213
 Tubb4a identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tubb4a
Molecular Function
    GTPase activity GO:0003924 IEA
    structural constituent of cytoskeleton GO:0005200 IEA
    GTP binding GO:0005525 IEA
Biological Process
    protein polymerization GO:0051258 IEA
    microtubule-based process GO:0007017 IEA
    metabolic process GO:0008152 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISO
    neuronal cell body GO:0043025 ISO
    cell projection GO:0042995 ISO
    extracellular exosome GO:0070062 ISO
    internode region of axon GO:0033269 ISO
    microtubule GO:0005874 ISO
    myelin sheath GO:0043209 ISO
    nucleus GO:0005634 ISO
    cilium GO:0005929 ISO
 Experiment description of studies that identified Tubb4a in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tubb4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tubb4a is involved
No pathways found





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