Gene description for Tubb4a
Gene name tubulin, beta 4A class IVa
Gene symbol Tubb4a
Other names/aliases Tubb4
Species Rattus norvegicus
 Database cross references - Tubb4a
ExoCarta ExoCarta_29213
Vesiclepedia VP_29213
Entrez Gene 29213
 Tubb4a identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Tubb4a
Molecular Function
    GTPase activity GO:0003924 IEA
    calcium ion binding GO:0005509 ISO
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    negative regulation of microtubule polymerization GO:0031115 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    microtubule GO:0005874 ISO
    cilium GO:0005929 ISO
    axoneme GO:0005930 ISO
    microtubule cytoskeleton GO:0015630 ISO
    motile cilium GO:0031514 IEA
    internode region of axon GO:0033269 ISO
    cell projection GO:0042995 ISO
    neuronal cell body GO:0043025 ISO
    myelin sheath GO:0043209 ISO
    intercellular bridge GO:0045171 IEA
    intercellular bridge GO:0045171 ISO
    mitotic spindle GO:0072686 IEA
    mitotic spindle GO:0072686 ISO
 Experiment description of studies that identified Tubb4a in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tubb4a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Gja1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Tubb4a is involved
No pathways found





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