Experiment description of studies that identified F2 in exosomes |
1 |
Experiment ID |
35 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
TSG101|Alix
|
EV Cytosolic markers |
✔
CD81|CD63
|
EV Membrane markers |
✔
HSPA5
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19367702
|
Organism |
Rattus norvegicus |
Experiment description |
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. |
Authors |
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM |
Journal name |
JPR
|
Publication year |
2008 |
Sample |
Hepatocytes |
Sample name |
Hepatocytes |
Isolation/purification methods |
Differential centrifugation Filtration Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting |
|
|
2 |
Experiment ID |
92 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|GAPDH
|
EV Cytosolic markers |
✔
CD9|CD151
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mrna
|
Identification method |
Microarray
|
PubMed ID |
20124479
|
Organism |
Rattus norvegicus |
Experiment description |
Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. |
Authors |
Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. |
Journal name |
CR
|
Publication year |
2010 |
Sample |
Pancreatic cancer cells |
Sample name |
AS |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14-1.17 g/mL
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-PCR Western blotting RatRef-12 Microarray FACS Mass spectrometry [MALDI TOF] |
|
|
3 |
Experiment ID |
93 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|GAPDH
|
EV Cytosolic markers |
✔
CD9|CD151
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mrna
|
Identification method |
Microarray
|
PubMed ID |
20124479
|
Organism |
Rattus norvegicus |
Experiment description |
Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation. |
Authors |
Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M. |
Journal name |
CR
|
Publication year |
2010 |
Sample |
Pancreatic cancer cells |
Sample name |
AS-Tspan8 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.14-1.17 g/mL
|
Molecules identified in the study |
Protein mRNA |
Methods used in the study |
RT-PCR Western blotting |
|
|