Gene description for Rps14
Gene name ribosomal protein S14
Gene symbol Rps14
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Rps14
ExoCarta ExoCarta_29284
Vesiclepedia VP_29284
Entrez Gene 29284
UniProt P13471  
 Rps14 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Rps14
Molecular Function
    RNA binding GO:0003723 ISS
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 IMP
    structural constituent of ribosome GO:0003735 ISO
    translation regulator activity GO:0045182 ISO
    mRNA 5'-UTR binding GO:0048027 ISO
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    ribosomal small subunit assembly GO:0000028 IMP
    ribosomal small subunit assembly GO:0000028 ISS
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    cytoplasmic translation GO:0002181 ISO
    translation GO:0006412 IBA
    translation GO:0006412 IEA
    translation GO:0006412 ISO
    regulation of translation GO:0006417 IEA
    erythrocyte differentiation GO:0030218 ISO
    maturation of SSU-rRNA GO:0030490 ISS
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
Subcellular Localization
    nucleolus GO:0005730 ISO
    ribosome GO:0005840 IEA
    postsynaptic density GO:0014069 ISO
    small ribosomal subunit GO:0015935 IEA
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    synapse GO:0045202 ISO
    ribonucleoprotein complex GO:1990904 IEA
 Experiment description of studies that identified Rps14 in sEVs
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Rps14
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Snca  
Affinity Capture-MS Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
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