Gene description for Ruvbl2
Gene name RuvB-like AAA ATPase 2
Gene symbol Ruvbl2
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Ruvbl2
ExoCarta ExoCarta_292907
Entrez Gene 292907
 Ruvbl2 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Ruvbl2
Molecular Function
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    molecular_function GO:0003674 ND
    ATPase activity GO:0016887 ISO
    DNA helicase activity GO:0003678 ISO
    ATP-dependent 5'-3' DNA helicase activity GO:0043141 IEA
    chromatin DNA binding GO:0031490 ISO
    identical protein binding GO:0042802 ISO
    damaged DNA binding GO:0003684 IEA
    ATP binding GO:0005524 IEA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 ISO
Biological Process
    cellular response to estradiol stimulus GO:0071392 ISO
    histone H4 acetylation GO:0043967 ISO
    biological_process GO:0008150 ND
    chromatin remodeling GO:0006338 ISO
    cellular response to UV GO:0034644 ISO
    establishment of protein localization to chromatin GO:0071169 ISO
    negative regulation of estrogen receptor binding GO:0071899 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    DNA duplex unwinding GO:0032508 ISO
    histone H2A acetylation GO:0043968 ISO
    DNA repair GO:0006281 IEA
    positive regulation of histone acetylation GO:0035066 ISO
    transcriptional activation by promoter-enhancer looping GO:0071733 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    MLL1 complex GO:0071339 ISO
    cellular_component GO:0005575 ND
    ribonucleoprotein complex GO:0030529 ISO
    NuA4 histone acetyltransferase complex GO:0035267 ISO
    Ino80 complex GO:0031011 ISO
    extracellular exosome GO:0070062 ISO
    intracellular GO:0005622 ISO
    Swr1 complex GO:0000812 ISO
    cytoplasm GO:0005737 ISO
    nuclear euchromatin GO:0005719 ISO
    nucleoplasm GO:0005654 ISO
 Experiment description of studies that identified Ruvbl2 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Ruvbl2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Ruvbl2 is involved
No pathways found





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