Gene description for Tsg101
Gene name tumor susceptibility 101
Gene symbol Tsg101
Other names/aliases Rw
Species Rattus norvegicus
 Database cross references - Tsg101
ExoCarta ExoCarta_292925
Entrez Gene 292925
UniProt Q6IRE4  
 Tsg101 identified in exosomes derived from the following tissue/cell type
Cortical neurones 16446100    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tsg101
Molecular Function
    transcription corepressor activity GO:0003714 ISO
    virion binding GO:0046790 ISO
    ligand-dependent nuclear receptor transcription coactivator activity GO:0030374 IDA
    protein homodimerization activity GO:0042803 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    calcium-dependent protein binding GO:0048306 ISO
    ubiquitin binding GO:0043130 ISO
Biological Process
    cell differentiation GO:0030154 ISO
    keratinocyte differentiation GO:0030216 ISO
    positive regulation of exosomal secretion GO:1903543 ISO
    viral budding GO:0046755 ISS
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    regulation of extracellular exosome assembly GO:1903551 ISO
    regulation of MAP kinase activity GO:0043405 ISO
    regulation of viral budding via host ESCRT complex GO:1903772 ISO
    cellular protein modification process GO:0006464 IEA
    cell division GO:0051301 IEA
    regulation of nucleic acid-templated transcription GO:1903506 IDA
    regulation of growth GO:0040008 IEA
    regulation of cell growth GO:0001558 ISO
    cell cycle arrest GO:0007050 ISO
    negative regulation of cell proliferation GO:0008285 ISO
    protein monoubiquitination GO:0006513 IDA
    regulation of viral release from host cell GO:1902186 ISO
    protein transport GO:0015031 IEA
    positive regulation of viral process GO:0048524 ISO
    endosome to lysosome transport GO:0008333 IMP
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISS
    negative regulation of transcription, DNA-templated GO:0045892 ISO
    positive regulation of viral release from host cell GO:1902188 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    late endosome GO:0005770 ISO
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISS
    early endosome GO:0005769 ISO
    endosome membrane GO:0010008 IDA
    ESCRT I complex GO:0000813 ISS
    late endosome membrane GO:0031902 IEA
    endosome GO:0005768 ISO
    plasma membrane GO:0005886 ISO
 Experiment description of studies that identified Tsg101 in exosomes
1
Experiment ID 30
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 35
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD81|CD63
EV Membrane markers
HSPA5
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tsg101
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AATF  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which Tsg101 is involved
No pathways found





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