Gene description for Tsg101
Gene name
tumor susceptibility 101
Gene symbol
Tsg101
Other names/aliases
Rw
Species
Rattus norvegicus
Database cross references - Tsg101
ExoCarta
ExoCarta_292925
Entrez Gene
292925
UniProt
Q6IRE4
Tsg101 identified in exosomes derived from the following tissue/cell type
Cortical neurones
16446100
Hepatocytes
19367702
Reticulocytes
21828046
Reticulocytes
21828046
Reticulocytes
21828046
Gene ontology annotations for Tsg101
Experiment description of studies that identified Tsg101 in exosomes
1
Experiment ID
30
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
FLOT
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
16446100
Organism
Rattus norvegicus
Experiment description
Exosomes are released by cultured cortical neurones.
Authors
Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name
MCN
Publication year
2006
Sample
Cortical neurones
Sample name
Cortical neurones
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.13 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting
2
Experiment ID
35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
19367702
Organism
Rattus norvegicus
Experiment description
Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors
Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name
JPR
Publication year
2008
Sample
Hepatocytes
Sample name
Hepatocytes
Isolation/purification methods
Differential centrifugation Filtration Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF] Western blotting
3
Experiment ID
96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap] Western blotting
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D2 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
4
Experiment ID
97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap] Western blotting
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D4 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.16-1.21 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
5
Experiment ID
98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [Orbitrap]
PubMed ID
21828046
Organism
Rattus norvegicus
Experiment description
The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors
Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name
JBC
Publication year
2011
Sample
Reticulocytes
Sample name
Reticulocytes - D7 Rep 2
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.25 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting
Protein-protein interactions for Tsg101
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
AATF
Two-hybrid
Homo sapiens
Reconstituted Complex
Homo sapiens
View the network
image/svg+xml
Pathways in which Tsg101 is involved
No pathways found