Gene description for Tsg101
Gene name tumor susceptibility 101
Gene symbol Tsg101
Other names/aliases Rw
Species Rattus norvegicus
 Database cross references - Tsg101
ExoCarta ExoCarta_292925
Vesiclepedia VP_292925
Entrez Gene 292925
UniProt Q6IRE4  
 Tsg101 identified in sEVs derived from the following tissue/cell type
Cortical neurones 16446100    
Hepatocytes 19367702    
Hippocampal neuronal cells 34654817    
Hippocampal neuronal cells 34654817    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tsg101
Molecular Function
    transcription corepressor activity GO:0003714 IEA
    transcription corepressor activity GO:0003714 ISO
    nuclear receptor coactivator activity GO:0030374 IDA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein homodimerization activity GO:0042803 IEA
    protein homodimerization activity GO:0042803 ISO
    ubiquitin binding GO:0043130 IBA
    ubiquitin binding GO:0043130 IEA
    ubiquitin binding GO:0043130 ISO
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
    virion binding GO:0046790 IEA
    virion binding GO:0046790 ISO
    calcium-dependent protein binding GO:0048306 IEA
    calcium-dependent protein binding GO:0048306 ISO
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    negative regulation of transcription by RNA polymerase II GO:0000122 ISO
    regulation of cell growth GO:0001558 IEA
    regulation of cell growth GO:0001558 ISO
    protein monoubiquitination GO:0006513 IDA
    extracellular transport GO:0006858 IEA
    extracellular transport GO:0006858 ISO
    negative regulation of epidermal growth factor-activated receptor activity GO:0007175 ISO
    negative regulation of cell population proliferation GO:0008285 IEA
    negative regulation of cell population proliferation GO:0008285 ISO
    endosome to lysosome transport GO:0008333 IBA
    endosome to lysosome transport GO:0008333 IMP
    protein transport GO:0015031 IEA
    viral release from host cell GO:0019076 IEA
    cell differentiation GO:0030154 ISO
    keratinocyte differentiation GO:0030216 IEA
    keratinocyte differentiation GO:0030216 ISO
    protein modification process GO:0036211 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 IEA
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 IEA
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISS
    regulation of MAP kinase activity GO:0043405 ISO
    positive regulation of DNA-templated transcription GO:0045893 IEA
    viral budding GO:0046755 IEA
    viral budding GO:0046755 ISO
    viral budding GO:0046755 ISS
    cell division GO:0051301 IEA
    regulation of cell cycle GO:0051726 IEA
    regulation of cell cycle GO:0051726 ISO
    positive regulation of exosomal secretion GO:1903543 IEA
    positive regulation of exosomal secretion GO:1903543 ISO
    regulation of extracellular exosome assembly GO:1903551 IEA
    regulation of extracellular exosome assembly GO:1903551 ISO
    positive regulation of viral budding via host ESCRT complex GO:1903774 IEA
    positive regulation of viral budding via host ESCRT complex GO:1903774 ISO
    exosomal secretion GO:1990182 IEA
    exosomal secretion GO:1990182 ISO
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 IEA
    positive regulation of ubiquitin-dependent endocytosis GO:2000397 ISO
Subcellular Localization
    ESCRT I complex GO:0000813 IBA
    ESCRT I complex GO:0000813 IEA
    ESCRT I complex GO:0000813 ISO
    ESCRT I complex GO:0000813 ISS
    nucleus GO:0005634 IEA
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    endosome GO:0005768 IEA
    endosome GO:0005768 ISO
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    late endosome GO:0005770 IEA
    late endosome GO:0005770 ISO
    centrosome GO:0005813 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endosome membrane GO:0010008 IDA
    early endosome membrane GO:0031901 IEA
    late endosome membrane GO:0031902 IEA
    extracellular exosome GO:0070062 IEA
    extracellular exosome GO:0070062 ISO
    Flemming body GO:0090543 IEA
 Experiment description of studies that identified Tsg101 in sEVs
1
Experiment ID 30
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors "Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R"
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
2
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 1160
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
4
Experiment ID 1161
MISEV standards
✘ Biophysical techniques
✔
Cd63|Tsg101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Western blotting
PubMed ID 34654817    
Organism Rattus norvegicus
Experiment description Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors
Authors "Xia W, Xie J, Cai Z, Liu X, Wen J, Cui ZK, Zhao R, Zhou X, Chen J, Mao X, Gu Z, Zou Z, Zou Z, Zhang Y, Zhao M, Mac M, Song Q, Bai X."
Journal name Nat Commun
Publication year 2021
Sample Hippocampal neuronal cells
Sample name Primary hippocampal neurons - Sprague-Dawley rat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
5
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
Western blotting
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
7
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tsg101
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Aatf  
Two-hybrid Rattus norvegicus
2 AATF  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
3 Scnn1g  
Reconstituted Complex Rattus norvegicus
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