Experiment description of studies that identified Tsg101 in exosomes

1 Experiment ID 30 ISEV standards ✔ EM EV Biophysical techniques ✔ Alix|TSG101 EV Cytosolic markers ✔ FLOT EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 16446100     Organism Rattus norvegicus Experiment description Exosomes are released by cultured cortical neurones. Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R Journal name MCN Publication year 2006 Sample Cortical neurones Sample name Cortical neurones Isolation/purification methods Differential centrifugation Sucrose density gradient Flotation density 1.13 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting

2 Experiment ID 35 ISEV standards ✔ CEM EV Biophysical techniques ✔ TSG101|Alix EV Cytosolic markers ✔ CD81|CD63 EV Membrane markers ✔ HSPA5 EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Western blotting PubMed ID 19367702     Organism Rattus norvegicus Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes. Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM Journal name JPR Publication year 2008 Sample Hepatocytes Sample name Hepatocytes Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [QTOF] Western blotting

3 Experiment ID 96 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] Western blotting PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D2 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

4 Experiment ID 97 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] Western blotting PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D4 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.16-1.21 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

5 Experiment ID 98 ISEV standards ✔ EM EV Biophysical techniques ✔ TSG101|HSP90|HSC70 EV Cytosolic markers ✔ MHCI EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry [Orbitrap] PubMed ID 21828046     Organism Rattus norvegicus Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation. Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E. Journal name JBC Publication year 2011 Sample Reticulocytes Sample name Reticulocytes - D7 Rep 2 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.25 g/mL Molecules identified in the study Protein Lipids Methods used in the study Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting

Protein-protein interactions for Tsg101

Protein Interactor ExoCarta ID Identification method PubMed Species 1 AATF   Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens View the network

Pathways in which Tsg101 is involved

No pathways found