Gene description for Gpx4
Gene name glutathione peroxidase 4
Gene symbol Gpx4
Other names/aliases Gshpx-4
Phgpx
gpx-4
snGpx
Species Rattus norvegicus
 Database cross references - Gpx4
ExoCarta ExoCarta_29328
Vesiclepedia VP_29328
Entrez Gene 29328
UniProt P36970   Q91XR8  
 Gpx4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Gpx4
Molecular Function
    glutathione peroxidase activity GO:0004602 IBA
    glutathione peroxidase activity GO:0004602 ISO
    glutathione peroxidase activity GO:0004602 ISS
    selenium binding GO:0008430 IDA
    identical protein binding GO:0042802 ISO
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 IBA
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 IDA
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 ISO
Biological Process
    chromatin organization GO:0006325 ISO
    chromatin organization GO:0006325 ISS
    apoptotic process GO:0006915 ISO
    response to oxidative stress GO:0006979 ISO
    response to oxidative stress GO:0006979 ISS
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISO
    spermatogenesis GO:0007283 ISS
    dendrite development GO:0016358 ISO
    arachidonate metabolic process GO:0019369 ISO
    arachidonate metabolic process GO:0019369 ISS
    lipoxygenase pathway GO:0019372 ISO
    lipoxygenase pathway GO:0019372 ISS
    cerebellum development GO:0021549 ISO
    response to estradiol GO:0032355 IEP
    response to lipopolysaccharide GO:0032496 ISO
    multicellular organism growth GO:0035264 ISO
    protein polymerization GO:0051258 ISO
    cellular oxidant detoxification GO:0098869 IEA
    negative regulation of ferroptosis GO:0110076 ISO
    negative regulation of ferroptosis GO:0110076 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    nuclear envelope GO:0005635 ISO
    nucleolus GO:0005730 IEA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    protein-containing complex GO:0032991 ISO
 Experiment description of studies that identified Gpx4 in exosomes
1
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gpx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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