Gene description for Gpx4
Gene name glutathione peroxidase 4
Gene symbol Gpx4
Other names/aliases Gshpx-4
Phgpx
gpx-4
snGpx
Species Rattus norvegicus
 Database cross references - Gpx4
ExoCarta ExoCarta_29328
Entrez Gene 29328
UniProt P36970   Q91XR8  
 Gpx4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Gpx4
Molecular Function
    selenium binding GO:0008430 IMP
    phospholipid-hydroperoxide glutathione peroxidase activity GO:0047066 IDA
    glutathione binding GO:0043295 IDA
    glutathione peroxidase activity GO:0004602 ISS
Biological Process
    spermatogenesis GO:0007283 ISS
    multicellular organismal development GO:0007275 IEA
    regulation of inflammatory response GO:0050727 IMP
    glutathione metabolic process GO:0006749 IDA
    response to oxidative stress GO:0006979 IEA
    hydrogen peroxide catabolic process GO:0042744 IDA
    response to estradiol GO:0032355 IEP
    aging GO:0007568 IEP
    oxidation-reduction process GO:0055114 ISO
    chromatin organization GO:0006325 ISS
Subcellular Localization
    nuclear envelope GO:0005635 ISO
    extracellular exosome GO:0070062 ISO
    mitochondrion GO:0005739 ISO
    cytosol GO:0005829 ISO
    mitochondrial inner membrane GO:0005743 ISO
    nucleus GO:0005634 ISS
 Experiment description of studies that identified Gpx4 in exosomes
1
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 192
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
CD63|CD81|FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Gpx4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Gpx4 is involved
PathwayEvidenceSource
Synthesis of 12-eicosatetraenoic acid derivatives IEA Reactome
Synthesis of 5-eicosatetraenoic acids IEA Reactome





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