Gene description for Tufm
Gene name Tu translation elongation factor, mitochondrial
Gene symbol Tufm
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tufm
ExoCarta ExoCarta_293481
Entrez Gene 293481
UniProt P85834  
 Tufm identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Tufm
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    GTP binding GO:0005525 IEA
    translation elongation factor activity GO:0003746 ISO
    GTPase activity GO:0003924 IEA
Biological Process
    translational elongation GO:0006414 ISO
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    mitochondrial nucleoid GO:0042645 ISO
    membrane GO:0016020 ISO
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrion GO:0005739 ISO
    myelin sheath GO:0043209 ISO
 Experiment description of studies that identified Tufm in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tufm
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tufm is involved
PathwayEvidenceSource
Mitochondrial translation elongation IEA Reactome





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