Gene description for Prkacb
Gene name protein kinase, cAMP dependent, catalytic, beta
Gene symbol Prkacb
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prkacb
ExoCarta ExoCarta_293508
Entrez Gene 293508
UniProt P68182  
 Prkacb identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Prkacb
Molecular Function
    ATP binding GO:0005524 ISS
    magnesium ion binding GO:0000287 ISS
    cAMP-dependent protein kinase activity GO:0004691 ISS
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A regulatory subunit binding GO:0034237 IC
Biological Process
    neural tube closure GO:0001843 ISO
    negative regulation of meiotic cell cycle GO:0051447 IDA
    response to clozapine GO:0097338 IEP
    negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning GO:1901621 ISO
    protein phosphorylation GO:0006468 ISS
    regulation of protein processing GO:0070613 ISO
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    plasma membrane GO:0005886 IEA
    cytoplasm GO:0005737 IDA
    extracellular exosome GO:0070062 ISO
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cytosol GO:0005829 TAS
    centrosome GO:0005813 ISO
    nucleus GO:0005634 IDA
    ciliary base GO:0097546 ISO
 Experiment description of studies that identified Prkacb in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkacb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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