Gene description for Prkacb
Gene name protein kinase, cAMP dependent, catalytic, beta
Gene symbol Prkacb
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prkacb
ExoCarta ExoCarta_293508
Vesiclepedia VP_293508
Entrez Gene 293508
UniProt P68182  
 Prkacb identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Prkacb
Molecular Function
    magnesium ion binding GO:0000287 IEA
    magnesium ion binding GO:0000287 ISO
    magnesium ion binding GO:0000287 ISS
    protein serine/threonine kinase activity GO:0004674 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    cAMP-dependent protein kinase activity GO:0004691 IBA
    cAMP-dependent protein kinase activity GO:0004691 IDA
    cAMP-dependent protein kinase activity GO:0004691 IEA
    cAMP-dependent protein kinase activity GO:0004691 ISO
    cAMP-dependent protein kinase activity GO:0004691 ISS
    ATP binding GO:0005524 IEA
    ATP binding GO:0005524 ISO
    ATP binding GO:0005524 ISS
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    protein kinase A regulatory subunit binding GO:0034237 IC
    protein serine kinase activity GO:0106310 IEA
Biological Process
    neural tube closure GO:0001843 IEA
    neural tube closure GO:0001843 ISO
    protein phosphorylation GO:0006468 IDA
    protein phosphorylation GO:0006468 ISO
    protein phosphorylation GO:0006468 ISS
    signal transduction GO:0007165 IBA
    negative regulation of smoothened signaling pathway GO:0045879 IEA
    negative regulation of smoothened signaling pathway GO:0045879 ISO
    negative regulation of meiotic cell cycle GO:0051447 IDA
    regulation of protein processing GO:0070613 IEA
    regulation of protein processing GO:0070613 ISO
    negative regulation of TORC1 signaling GO:1904262 IEA
    negative regulation of TORC1 signaling GO:1904262 ISO
    negative regulation of TORC1 signaling GO:1904262 ISS
Subcellular Localization
    nucleus GO:0005634 IBA
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    membrane GO:0016020 IEA
    perinuclear region of cytoplasm GO:0048471 IDA
    ciliary base GO:0097546 IEA
    ciliary base GO:0097546 ISO
 Experiment description of studies that identified Prkacb in sEVs
1
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Prkacb
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkacb is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Adaptive Immune System IEA Reactome
Aquaporin-mediated transport IEA Reactome
Axon guidance IEA Reactome
Ca-dependent events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
DAG and IP3 signaling IEA Reactome
DARPP-32 events IEA Reactome
Developmental Biology IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G-protein mediated events IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Glucagon signaling in metabolic regulation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
HDL assembly IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Immune System IEA Reactome
Integration of energy metabolism IEA Reactome
Intracellular signaling by second messengers IEA Reactome
MAPK family signaling cascades IEA Reactome
MAPK6/MAPK4 signaling IEA Reactome
Metabolism IEA Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
PKA activation IEA Reactome
PKA activation in glucagon signalling IEA Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
Plasma lipoprotein assembly IEA Reactome
Plasma lipoprotein assembly, remodeling, and clearance IEA Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
Rap1 signalling IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
RET signaling IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Hedgehog IEA Reactome
Signaling by Receptor Tyrosine Kinases IEA Reactome
Signaling by VEGF IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome
VEGFA-VEGFR2 Pathway IEA Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here