Gene description for Ehd1
Gene name EH-domain containing 1
Gene symbol Ehd1
Other names/aliases RGD1306960
Species Rattus norvegicus
 Database cross references - Ehd1
ExoCarta ExoCarta_293692
Vesiclepedia VP_293692
Entrez Gene 293692
UniProt Q641Z6  
 Ehd1 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Ehd1
Molecular Function
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    GTP binding GO:0005525 IEA
    small GTPase binding GO:0031267 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    intracellular protein transport GO:0006886 ISO
    intracellular protein transport GO:0006886 ISS
    endocytosis GO:0006897 IBA
    endocytosis GO:0006897 ISO
    endocytosis GO:0006897 ISO
    endocytosis GO:0006897 ISS
    positive regulation of cholesterol storage GO:0010886 ISO
    positive regulation of neuron projection development GO:0010976 IMP
    endosomal transport GO:0016197 ISO
    neuron projection development GO:0031175 IMP
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 ISO
    endocytic recycling GO:0032456 ISS
    low-density lipoprotein particle clearance GO:0034383 ISO
    cholesterol homeostasis GO:0042632 ISO
    protein homooligomerization GO:0051260 ISO
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 ISO
    cilium assembly GO:0060271 ISS
    protein localization to cilium GO:0061512 ISO
    protein localization to cilium GO:0061512 ISS
    protein localization to plasma membrane GO:0072659 IBA
    positive regulation of myoblast fusion GO:1901741 ISO
    positive regulation of myoblast fusion GO:1901741 ISS
    cellular response to nerve growth factor stimulus GO:1990090 IDA
    positive regulation of endocytic recycling GO:2001137 ISO
    positive regulation of endocytic recycling GO:2001137 ISS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    endosome GO:0005768 ISO
    early endosome GO:0005769 IBA
    early endosome GO:0005769 ISO
    lipid droplet GO:0005811 ISO
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 ISO
    plasma membrane GO:0005886 ISS
    endosome membrane GO:0010008 IDA
    ciliary pocket membrane GO:0020018 ISO
    ciliary pocket membrane GO:0020018 ISS
    endocytic vesicle GO:0030139 IBA
    endocytic vesicle GO:0030139 ISO
    platelet dense tubular network membrane GO:0031095 ISO
    early endosome membrane GO:0031901 ISO
    early endosome membrane GO:0031901 ISS
    perinuclear region of cytoplasm GO:0048471 IBA
    perinuclear region of cytoplasm GO:0048471 ISO
    presynaptic active zone GO:0048786 IDA
    recycling endosome membrane GO:0055038 IBA
    recycling endosome membrane GO:0055038 ISO
    recycling endosome membrane GO:0055038 ISS
    glutamatergic synapse GO:0098978 IDA
 Experiment description of studies that identified Ehd1 in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Ehd1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Igf1r  
Affinity Capture-Western Rattus norvegicus
2 Snap29  
Affinity Capture-Western Rattus norvegicus
View the network image/svg+xml
 Pathways in which Ehd1 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production IEA Reactome
Hemostasis IEA Reactome





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