Gene description for H2afy
Gene name H2A histone family, member Y
Gene symbol H2afy
Other names/aliases -
Species Rattus norvegicus
 Database cross references - H2afy
ExoCarta ExoCarta_29384
Entrez Gene 29384
UniProt Q02874  
 H2afy identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for H2afy
Molecular Function
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 ISO
    chromatin binding GO:0003682 ISO
    nucleosomal DNA binding GO:0031492 ISO
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 ISO
    molecular_function GO:0003674 ND
    protein heterodimerization activity GO:0046982 IEA
    rDNA binding GO:0000182 ISO
    transcription regulatory region DNA binding GO:0044212 ISO
    protein kinase binding GO:0019901 ISO
    core promoter sequence-specific DNA binding GO:0001046 ISO
    chromatin DNA binding GO:0031490 ISO
    protein serine/threonine kinase inhibitor activity GO:0030291 ISO
    double-stranded methylated DNA binding GO:0010385 ISO
Biological Process
    regulation of chromatin silencing at rDNA GO:0061187 ISO
    regulation of cell growth GO:0001558 ISO
    regulation of gene expression, epigenetic GO:0040029 ISO
    positive regulation of keratinocyte differentiation GO:0045618 ISO
    negative regulation of histone phosphorylation GO:0033128 ISO
    biological_process GO:0008150 ND
    negative regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter GO:1901837 ISO
    negative regulation of cell cycle G2/M phase transition GO:1902750 ISO
    dosage compensation GO:0007549 ISO
    positive regulation of gene expression, epigenetic GO:0045815 ISO
    chromatin modification GO:0016568 IEA
    negative regulation of gene expression, epigenetic GO:0045814 ISO
    establishment of protein localization to chromatin GO:0071169 ISO
    nucleosome assembly GO:0006334 IEA
    regulation of lipid metabolic process GO:0019216 ISO
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 ISO
    negative regulation of histone H3-K4 methylation GO:0051572 ISO
    negative regulation of protein serine/threonine kinase activity GO:0071901 ISO
    negative regulation of histone H3-K27 methylation GO:0061086 ISO
Subcellular Localization
    Barr body GO:0001740 ISO
    nucleosome GO:0000786 IDA
    nucleolus GO:0005730 ISO
    extracellular exosome GO:0070062 ISO
    condensed chromosome GO:0000793 ISO
    nucleoplasm GO:0005654 ISO
    pericentric heterochromatin GO:0005721 ISO
    chromatin GO:0000785 IDA
    ESC/E(Z) complex GO:0035098 ISO
    nuclear chromosome GO:0000228 ISO
    nuclear chromatin GO:0000790 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified H2afy in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 97
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for H2afy
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which H2afy is involved
No pathways found





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