Gene description for Bcap31
Gene name B-cell receptor-associated protein 31
Gene symbol Bcap31
Other names/aliases Bap31
Species Rattus norvegicus
 Database cross references - Bcap31
ExoCarta ExoCarta_293852
Vesiclepedia VP_293852
Entrez Gene 293852
 Bcap31 identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Bcap31
Molecular Function
    protein binding GO:0005515 IPI
    MHC class I protein binding GO:0042288 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    protein targeting to mitochondrion GO:0006626 ISO
    intracellular protein transport GO:0006886 IEA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 IBA
    endoplasmic reticulum to Golgi vesicle-mediated transport GO:0006888 ISO
    immune response GO:0006955 ISO
    spermatogenesis GO:0007283 ISO
    biological_process GO:0008150 ND
    response to endoplasmic reticulum stress GO:0034976 ISO
    protein localization to endoplasmic reticulum exit site GO:0070973 IBA
    protein localization to endoplasmic reticulum exit site GO:0070973 ISO
    positive regulation of retrograde protein transport, ER to cytosol GO:1904154 ISO
    positive regulation of ERAD pathway GO:1904294 ISO
    positive regulation of ubiquitin-dependent protein catabolic process GO:2000060 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
Subcellular Localization
    Golgi membrane GO:0000139 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IBA
    endoplasmic reticulum membrane GO:0005789 IDA
    lipid droplet GO:0005811 ISO
    plasma membrane GO:0005886 ISO
    clathrin-coated vesicle GO:0030136 IDA
    Golgi cisterna membrane GO:0032580 IDA
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 ISO
    perinuclear endoplasmic reticulum GO:0097038 ISO
 Experiment description of studies that identified Bcap31 in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bcap31
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Bcap31 is involved
No pathways found





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