Gene description for Bcap31
Gene name B-cell receptor-associated protein 31
Gene symbol Bcap31
Other names/aliases Bap31
Species Rattus norvegicus
 Database cross references - Bcap31
ExoCarta ExoCarta_293852
Entrez Gene 293852
 Bcap31 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
 Gene ontology annotations for Bcap31
Molecular Function
    protein binding GO:0005515 IPI
    MHC class I protein binding GO:0042288 ISO
    protein complex binding GO:0032403 ISO
Biological Process
    immune response GO:0006955 ISO
    spermatogenesis GO:0007283 IEA
    positive regulation of ER-associated ubiquitin-dependent protein catabolic process GO:1903071 ISO
    biological_process GO:0008150 ND
    ER to Golgi vesicle-mediated transport GO:0006888 ISO
    negative regulation of endoplasmic reticulum calcium ion concentration GO:0032471 ISO
    positive regulation of cytosolic calcium ion concentration GO:0007204 ISO
    protein localization to endoplasmic reticulum exit site GO:0070973 ISO
    positive regulation of retrograde protein transport, ER to cytosol GO:1904154 ISO
    positive regulation of mitochondrial calcium ion concentration GO:0051561 ISO
    positive regulation of intrinsic apoptotic signaling pathway GO:2001244 ISO
    positive regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043280 ISO
    intracellular protein transport GO:0006886 IEA
    calcium-mediated signaling using intracellular calcium source GO:0035584 ISO
Subcellular Localization
    lipid particle GO:0005811 ISO
    cytoplasm GO:0005737 ISO
    Golgi membrane GO:0000139 ISO
    Sec61 translocon complex GO:0005784 ISO
    plasma membrane GO:0005886 ISO
    intracellular GO:0005622 ISO
    mitochondrion GO:0005739 ISO
    cell GO:0005623 ISO
    Golgi cisterna membrane GO:0032580 IDA
    integral component of plasma membrane GO:0005887 ISO
    endoplasmic reticulum GO:0005783 ISO
    endoplasmic reticulum membrane GO:0005789 IDA
    membrane GO:0016020 ISO
    cytosol GO:0005829 IEA
    clathrin-coated vesicle GO:0030136 IDA
 Experiment description of studies that identified Bcap31 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Bcap31
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Bcap31 is involved
No pathways found





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