Gene description for Hspa1b
Gene name heat shock 70kD protein 1B (mapped)
Gene symbol Hspa1b
Other names/aliases Hsp70-2
Hsp72
Hspa2
Species Rattus norvegicus
 Database cross references - Hspa1b
ExoCarta ExoCarta_294254
Entrez Gene 294254
UniProt Q07439  
 Hspa1b identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Hepatocytes 19367702    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Hspa1b
Molecular Function
    ubiquitin protein ligase binding GO:0031625 ISO
    G-protein coupled receptor binding GO:0001664 ISO
    double-stranded RNA binding GO:0003725 ISO
    heat shock protein binding GO:0031072 ISO
    protein binding involved in protein folding GO:0044183 ISO
    C3HC4-type RING finger domain binding GO:0055131 ISO
    molecular_function GO:0003674 ND
    ATP binding GO:0005524 ISO
    protein N-terminus binding GO:0047485 ISO
    poly(A) RNA binding GO:0044822 ISO
    protein binding GO:0005515 IPI
    ATPase activity, coupled GO:0042623 ISO
    ATPase activity GO:0016887 ISO
    unfolded protein binding GO:0051082 ISO
    enzyme binding GO:0019899 ISO
Biological Process
    cellular heat acclimation GO:0070370 ISO
    negative regulation of cell growth GO:0030308 ISS
    negative regulation of cell death GO:0060548 ISO
    negative regulation of extrinsic apoptotic signaling pathway in absence of ligand GO:2001240 ISO
    response to mechanical stimulus GO:0009612 IEP
    positive regulation of gene expression GO:0010628 ISO
    response to ethanol GO:0045471 IEP
    positive regulation of neuron differentiation GO:0045666 IMP
    negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway GO:1902236 ISO
    negative regulation of cell proliferation GO:0008285 ISS
    response to unfolded protein GO:0006986 ISS
    response to ischemia GO:0002931 IEP
    regulation of cell death GO:0010941 ISO
    negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway GO:1901029 ISO
    negative regulation of inclusion body assembly GO:0090084 ISO
    positive regulation of mRNA cleavage GO:0031439 ISO
    protein refolding GO:0042026 ISO
    DNA repair GO:0006281 ISO
    neuron differentiation GO:0030182 IEP
    mRNA catabolic process GO:0006402 ISS
    telomere maintenance GO:0000723 ISO
    positive regulation of endoribonuclease activity GO:1902380 ISO
    cellular response to heat GO:0034605 ISO
    response to heat GO:0009408 ISO
    negative regulation of apoptotic process GO:0043066 ISO
    negative regulation of protein ubiquitination GO:0031397 ISO
    positive regulation of erythrocyte differentiation GO:0045648 ISO
    ATP metabolic process GO:0046034 ISO
    response to radiation GO:0009314 IEP
    positive regulation of IRE1-mediated unfolded protein response GO:1903896 ISO
Subcellular Localization
    nuclear speck GO:0016607 ISS
    ribonucleoprotein complex GO:0030529 ISO
    nucleus GO:0005634 ISS
    perinuclear region of cytoplasm GO:0048471 ISS
    inclusion body GO:0016234 ISO
    COP9 signalosome GO:0008180 ISO
    vesicle GO:0031982 ISO
    aggresome GO:0016235 ISO
    cellular_component GO:0005575 ND
    extracellular exosome GO:0070062 ISO
    centriole GO:0005814 ISO
    cytosol GO:0005829 ISO
    ubiquitin ligase complex GO:0000151 ISO
    focal adhesion GO:0005925 ISO
    cytoplasm GO:0005737 ISS
    blood microparticle GO:0072562 ISO
 Experiment description of studies that identified Hspa1b in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 35
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD81|CD63
EV Membrane markers
✔
HSPA5
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
3
Experiment ID 94
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
5
Experiment ID 96
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
6
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Hspa1b
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Hspa1b is involved
No pathways found





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