Gene description for Wasf1
Gene name WAS protein family, member 1
Gene symbol Wasf1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Wasf1
ExoCarta ExoCarta_294568
Vesiclepedia VP_294568
Entrez Gene 294568
UniProt Q5BJU7  
 Wasf1 identified in sEVs derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Wasf1
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IEA
    small GTPase binding GO:0031267 ISO
    protein kinase A regulatory subunit binding GO:0034237 IBA
    protein kinase A binding GO:0051018 IEA
    protein kinase A binding GO:0051018 ISO
    Arp2/3 complex binding GO:0071933 IBA
Biological Process
    receptor-mediated endocytosis GO:0006898 IEA
    receptor-mediated endocytosis GO:0006898 ISO
    receptor-mediated endocytosis GO:0006898 ISS
    mitochondrion organization GO:0007005 IEA
    mitochondrion organization GO:0007005 ISO
    mitochondrion organization GO:0007005 ISS
    Rac protein signal transduction GO:0016601 IEA
    Rac protein signal transduction GO:0016601 ISO
    actin cytoskeleton organization GO:0030036 IBA
    actin cytoskeleton organization GO:0030036 IEA
    actin cytoskeleton organization GO:0030036 ISO
    actin cytoskeleton organization GO:0030036 ISS
    neuron projection development GO:0031175 ISO
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 IEA
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 ISO
    lamellipodium morphogenesis GO:0072673 IEA
    lamellipodium morphogenesis GO:0072673 ISO
    lamellipodium morphogenesis GO:0072673 ISO
    lamellipodium morphogenesis GO:0072673 ISS
    dendrite extension GO:0097484 IEA
    dendrite extension GO:0097484 ISO
    modification of postsynaptic actin cytoskeleton GO:0098885 IEA
    modification of postsynaptic actin cytoskeleton GO:0098885 ISO
    dendritic transport of mitochondrion GO:0098939 IDA
    dendritic transport of mitochondrion GO:0098939 IMP
    cellular response to brain-derived neurotrophic factor stimulus GO:1990416 IEA
    cellular response to brain-derived neurotrophic factor stimulus GO:1990416 ISO
    cellular response to brain-derived neurotrophic factor stimulus GO:1990416 ISS
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IBA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 IEA
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial outer membrane GO:0005741 IEA
    mitochondrial outer membrane GO:0005741 ISO
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IEA
    lamellipodium GO:0030027 IBA
    lamellipodium GO:0030027 IEA
    lamellipodium GO:0030027 ISO
    SCAR complex GO:0031209 IBA
    SCAR complex GO:0031209 IEA
    SCAR complex GO:0031209 ISO
    dendrite cytoplasm GO:0032839 IEA
    protein-containing complex GO:0032991 ISO
    postsynapse GO:0098794 IEA
    postsynapse GO:0098794 ISO
 Experiment description of studies that identified Wasf1 in sEVs
1
Experiment ID 92
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
Biophysical techniques
HSP70|GAPDH|CD9|CD151
Enriched markers
Negative markers
Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors "Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M."
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Wasf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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