Gene description for Wasf1
Gene name WAS protein family, member 1
Gene symbol Wasf1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Wasf1
ExoCarta ExoCarta_294568
Entrez Gene 294568
UniProt Q5BJU7  
 Wasf1 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Wasf1
Molecular Function
    actin binding GO:0003779 IEA
    Rac GTPase binding GO:0048365 ISO
    protein complex binding GO:0032403 ISO
    protein binding GO:0005515 IPI
Biological Process
    Rac protein signal transduction GO:0016601 ISO
    positive regulation of Arp2/3 complex-mediated actin nucleation GO:2000601 ISO
    actin cytoskeleton organization GO:0030036 IEA
    lamellipodium morphogenesis GO:0072673 ISO
Subcellular Localization
    cytoplasm GO:0005737 ISO
    cytoskeleton GO:0005856 IEA
    lamellipodium GO:0030027 ISO
    synapse GO:0045202 IEA
    mitochondrial outer membrane GO:0005741 ISO
    SCAR complex GO:0031209 ISO
    cell junction GO:0030054 IEA
    mitochondrion GO:0005739 ISO
 Experiment description of studies that identified Wasf1 in exosomes
1
Experiment ID 92
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
ISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH
EV Cytosolic markers
CD9|CD151
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Wasf1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Wasf1 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here