Gene description for Slc1a3
Gene name solute carrier family 1 (glial high affinity glutamate transporter), member 3
Gene symbol Slc1a3
Other names/aliases EAAT1
GLAST
GLAST-1
GluT-1
Species Rattus norvegicus
 Database cross references - Slc1a3
ExoCarta ExoCarta_29483
Entrez Gene 29483
UniProt P24942  
 Slc1a3 identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Cortical neurones 16446100    
 Gene ontology annotations for Slc1a3
Molecular Function
    sodium:dicarboxylate symporter activity GO:0017153 IEA
    high-affinity glutamate transmembrane transporter activity GO:0005314 ISO
    L-glutamate transmembrane transporter activity GO:0005313 TAS
    glutamate binding GO:0016595 ISO
    amino acid binding GO:0016597 ISO
Biological Process
    neuromuscular process controlling balance GO:0050885 ISO
    cell morphogenesis involved in neuron differentiation GO:0048667 ISO
    L-glutamate transport GO:0015813 ISO
    neurotransmitter transport GO:0006836 TAS
    sodium ion transmembrane transport GO:0035725 ISO
    auditory behavior GO:0031223 ISO
    response to antibiotic GO:0046677 ISO
    glutamate metabolic process GO:0006536 ISO
    positive regulation of defense response to virus by host GO:0002230 ISO
    response to light stimulus GO:0009416 ISO
    gamma-aminobutyric acid biosynthetic process GO:0009449 ISO
    malate-aspartate shuttle GO:0043490 IDA
    response to inorganic substance GO:0010035 IDA
    glutamate biosynthetic process GO:0006537 ISO
    response to wounding GO:0009611 ISO
    sensory perception of sound GO:0007605 ISO
    cranial nerve development GO:0021545 ISO
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 ISO
    D-aspartate import GO:0070779 ISO
    response to drug GO:0042493 ISO
    L-glutamate import GO:0051938 ISO
    L-glutamate transmembrane transport GO:0089711 TAS
    positive regulation of synaptic transmission GO:0050806 ISO
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    neuronal cell body GO:0043025 ISO
    cell projection GO:0042995 ISO
    synapse GO:0045202 IDA
    dendritic spine GO:0043197 IDA
    cell periphery GO:0071944 ISO
    neuron projection GO:0043005 ISO
    plasma membrane GO:0005886 ISO
    mitochondrial inner membrane GO:0005743 IDA
    fibril GO:0043205 ISO
    basolateral plasma membrane GO:0016323 IDA
    cell surface GO:0009986 ISO
 Experiment description of studies that identified Slc1a3 in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 30
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101
EV Cytosolic markers
FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 16446100    
Organism Rattus norvegicus
Experiment description Exosomes are released by cultured cortical neurones.
Authors Faure J, Lachenal G, Court M, Hirrlinger J, Chatellard-Causse C, Blot B, Grange J, Schoehn G, Goldberg Y, Boyer V, Kirchhoff F, Raposo G, Garin J, Sadoul R
Journal name MCN
Publication year 2006
Sample Cortical neurones
Sample name Cortical neurones
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Slc1a3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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