Gene description for Gnb4
Gene name guanine nucleotide binding protein (G protein), beta polypeptide 4
Gene symbol Gnb4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Gnb4
ExoCarta ExoCarta_294962
Vesiclepedia VP_294962
Entrez Gene 294962
UniProt O35353  
 Gnb4 identified in sEVs derived from the following tissue/cell type
Hepatocytes 19367702    
 Gene ontology annotations for Gnb4
Molecular Function
    signaling receptor complex adaptor activity GO:0030159 IBA
    protein-containing complex binding GO:0044877 IEA
    protein-containing complex binding GO:0044877 ISO
Biological Process
    signal transduction GO:0007165 IEA
    G protein-coupled receptor signaling pathway GO:0007186 IBA
    substantia nigra development GO:0021762 ISO
Subcellular Localization
    cytoplasm GO:0005737 IBA
    heterotrimeric G-protein complex GO:0005834 IBA
    heterotrimeric G-protein complex GO:0005834 IEA
    heterotrimeric G-protein complex GO:0005834 ISO
    cell body GO:0044297 IDA
    synapse GO:0045202 IEA
    synapse GO:0045202 ISO
 Experiment description of studies that identified Gnb4 in sEVs
1
Experiment ID 35
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|CD81|CD63
Enriched markers
✔
HSPA5
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19367702    
Organism Rattus norvegicus
Experiment description Characterization and Comprehensive Proteome Profiling of Exosomes Secreted by Hepatocytes.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M, Elortza F, Lu SC, Mato JM, Falcon-Perez JM"
Journal name JPR
Publication year 2008
Sample Hepatocytes
Sample name Hepatocytes
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
Western blotting
 Protein-protein interactions for Gnb4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Gnb4 is involved
PathwayEvidenceSource
Activation of G protein gated Potassium channels IEA Reactome
Activation of GABAB receptors IEA Reactome
ADP signalling through P2Y purinoceptor 12 IEA Reactome
Adrenaline,noradrenaline inhibits insulin secretion IEA Reactome
Aquaporin-mediated transport IEA Reactome
Beta-catenin independent WNT signaling IEA Reactome
Ca2+ pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
Chaperonin-mediated protein folding IEA Reactome
Class B/2 (Secretin family receptors) IEA Reactome
Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (q) signalling events IEA Reactome
G alpha (z) signalling events IEA Reactome
G beta:gamma signalling through CDC42 IEA Reactome
G beta:gamma signalling through PI3Kgamma IEA Reactome
G protein gated Potassium channels IEA Reactome
G-protein activation IEA Reactome
G-protein beta:gamma signalling IEA Reactome
GABA B receptor activation IEA Reactome
GABA receptor activation IEA Reactome
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome
Glucagon-type ligand receptors IEA Reactome
GPCR downstream signalling IEA Reactome
GPCR ligand binding IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits IEA Reactome
Integration of energy metabolism IEA Reactome
Inwardly rectifying K+ channels IEA Reactome
Metabolism IEA Reactome
Metabolism of proteins IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
Platelet activation, signaling and aggregation IEA Reactome
Potassium Channels IEA Reactome
Protein folding IEA Reactome
Regulation of insulin secretion IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal amplification IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by WNT IEA Reactome
Thrombin signalling through proteinase activated receptors (PARs) IEA Reactome
Thromboxane signalling through TP receptor IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome





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