Gene description for Rap1a
Gene name RAP1A, member of RAS oncogene family
Gene symbol Rap1a
Other names/aliases RAP-1A
rap 1A
Species Rattus norvegicus
 Database cross references - Rap1a
ExoCarta ExoCarta_295347
Entrez Gene 295347
UniProt P62836  
 Rap1a identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Rap1a
Molecular Function
    Rap guanyl-nucleotide exchange factor activity GO:0017034 IMP
    protein transporter activity GO:0008565 ISS
    protein binding GO:0005515 IPI
    protein complex binding GO:0032403 ISS
    Ras GTPase binding GO:0017016 ISO
    GTPase activity GO:0003924 ISO
    GTP binding GO:0005525 IEA
Biological Process
    positive regulation of protein kinase activity GO:0045860 IMP
    cellular response to glucose stimulus GO:0071333 IEP
    small GTPase mediated signal transduction GO:0007264 ISO
    protein transport GO:0015031 ISS
    metabolic process GO:0008152 IEA
    positive regulation of vasculogenesis GO:2001214 ISS
    liver regeneration GO:0097421 IEP
    regulation of cell junction assembly GO:1901888 ISS
    cellular response to cAMP GO:0071320 ISS
    microvillus assembly GO:0030033 ISO
    nerve growth factor signaling pathway GO:0038180 IDA
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    positive regulation of neuron projection development GO:0010976 ISO
    Rap protein signal transduction GO:0032486 ISS
    negative regulation of synaptic vesicle exocytosis GO:2000301 ISO
    positive regulation of glucose import GO:0046326 IMP
    establishment of endothelial barrier GO:0061028 ISS
    cellular response to organic cyclic compound GO:0071407 IDA
    response to antineoplastic agent GO:0097327 IDA
    negative regulation of collagen biosynthetic process GO:0032966 IMP
    positive regulation of phagocytosis GO:0050766 IMP
    response to carbohydrate GO:0009743 IEP
    positive regulation of GTPase activity GO:0043547 IMP
    cellular response to nerve growth factor stimulus GO:1990090 IEP
    cellular response to drug GO:0035690 IDA
Subcellular Localization
    plasma membrane GO:0005886 ISO
    cytoplasm GO:0005737 ISS
    early endosome GO:0005769 IDA
    myelin sheath GO:0043209 ISO
    cytosol GO:0005829 TAS
    guanyl-nucleotide exchange factor complex GO:0032045 ISO
    neuron projection GO:0043005 ISO
    cell junction GO:0030054 ISS
    late endosome GO:0005770 IDA
    perinuclear region of cytoplasm GO:0048471 ISS
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Rap1a in exosomes
1
Experiment ID 225
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
ISEV standards
CEM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
CD63|CD81|FLOT
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rap1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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