Gene description for Rap1a
Gene name RAP1A, member of RAS oncogene family
Gene symbol Rap1a
Other names/aliases RAP-1A
rap 1A
Species Rattus norvegicus
 Database cross references - Rap1a
ExoCarta ExoCarta_295347
Vesiclepedia VP_295347
Entrez Gene 295347
UniProt P62836  
 Rap1a identified in sEVs derived from the following tissue/cell type
Adipocytes 25998041    
Urine 20535238    
 Gene ontology annotations for Rap1a
Molecular Function
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IEA
    GTPase activity GO:0003924 ISO
    G protein activity GO:0003925 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IDA
    GTP binding GO:0005525 IEA
    GDP binding GO:0019003 IBA
    small GTPase binding GO:0031267 ISO
    protein-containing complex binding GO:0044877 ISO
Biological Process
    signal transduction GO:0007165 IEA
    small GTPase-mediated signal transduction GO:0007264 ISO
    nervous system development GO:0007399 IEA
    response to carbohydrate GO:0009743 IEP
    positive regulation of neuron projection development GO:0010976 IMP
    positive regulation of neuron projection development GO:0010976 ISO
    synaptic vesicle exocytosis GO:0016079 ISO
    microvillus assembly GO:0030033 ISO
    Rap protein signal transduction GO:0032486 IBA
    Rap protein signal transduction GO:0032486 IEA
    Rap protein signal transduction GO:0032486 ISO
    Rap protein signal transduction GO:0032486 ISS
    negative regulation of collagen biosynthetic process GO:0032966 IMP
    nerve growth factor signaling pathway GO:0038180 IDA
    positive regulation of GTPase activity GO:0043547 IMP
    positive regulation of protein kinase activity GO:0045860 IMP
    positive regulation of D-glucose import GO:0046326 IMP
    positive regulation of phagocytosis GO:0050766 IMP
    positive regulation of Fc receptor mediated stimulatory signaling pathway GO:0060369 IMP
    establishment of endothelial barrier GO:0061028 ISO
    establishment of endothelial barrier GO:0061028 ISS
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 ISO
    cellular response to cAMP GO:0071320 IBA
    cellular response to cAMP GO:0071320 IEP
    cellular response to cAMP GO:0071320 ISO
    cellular response to cAMP GO:0071320 ISS
    cellular response to organic cyclic compound GO:0071407 IDA
    cellular response to xenobiotic stimulus GO:0071466 IDA
    protein localization to plasma membrane GO:0072659 ISO
    protein localization to plasma membrane GO:0072659 ISS
    response to antineoplastic agent GO:0097327 IDA
    liver regeneration GO:0097421 IEP
    regulation of neurotransmitter receptor localization to postsynaptic specialization membrane GO:0098696 ISO
    regulation of cell junction assembly GO:1901888 ISO
    regulation of cell junction assembly GO:1901888 ISS
    cellular response to nerve growth factor stimulus GO:1990090 IDA
    cellular response to nerve growth factor stimulus GO:1990090 IEP
    negative regulation of synaptic vesicle exocytosis GO:2000301 IBA
    negative regulation of synaptic vesicle exocytosis GO:2000301 ISO
    positive regulation of vasculogenesis GO:2001214 ISO
    positive regulation of vasculogenesis GO:2001214 ISS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    cytoplasm GO:0005737 ISS
    early endosome GO:0005769 IDA
    late endosome GO:0005770 IDA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    endomembrane system GO:0012505 IEA
    membrane GO:0016020 IEA
    cell junction GO:0030054 ISO
    cell junction GO:0030054 ISS
    guanyl-nucleotide exchange factor complex GO:0032045 ISO
    neuron projection GO:0043005 ISO
    phagocytic vesicle GO:0045335 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    perinuclear region of cytoplasm GO:0048471 ISO
    perinuclear region of cytoplasm GO:0048471 ISS
    anchoring junction GO:0070161 IEA
    sperm midpiece GO:0097225 ISO
    sperm midpiece GO:0097225 ISS
    presynapse GO:0098793 IEA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Rap1a in sEVs
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rap1a
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Mib1  
Affinity Capture-MS Rattus norvegicus
2 Sumo3  
Affinity Capture-MS Rattus norvegicus
3 Gja1  
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here