Gene description for Spc25
Gene name SPC25, NDC80 kinetochore complex component
Gene symbol Spc25
Other names/aliases RGD1307811
Spbc25
Species Rattus norvegicus
 Database cross references - Spc25
ExoCarta ExoCarta_295661
Vesiclepedia VP_295661
Entrez Gene 295661
UniProt Q5M856  
 Spc25 identified in exosomes derived from the following tissue/cell type
Urine 20535238    
 Gene ontology annotations for Spc25
Biological Process
    mitotic spindle organization GO:0007052 ISO
    mitotic spindle organization GO:0007052 ISS
    chromosome segregation GO:0007059 IBA
    chromosome segregation GO:0007059 ISO
    chromosome segregation GO:0007059 ISS
    attachment of spindle microtubules to kinetochore GO:0008608 ISO
    cell division GO:0051301 IEA
Subcellular Localization
    kinetochore GO:0000776 ISO
    kinetochore GO:0000776 ISS
    outer kinetochore GO:0000940 ISO
    nucleus GO:0005634 IEA
    cytosol GO:0005829 IEA
    cytosol GO:0005829 ISO
    Ndc80 complex GO:0031262 IBA
    Ndc80 complex GO:0031262 ISO
    Ndc80 complex GO:0031262 ISS
 Experiment description of studies that identified Spc25 in exosomes
1
Experiment ID 192
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Spc25
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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