Gene description for Atp2b4
Gene name ATPase, Ca++ transporting, plasma membrane 4
Gene symbol Atp2b4
Other names/aliases PMCA4
Species Rattus norvegicus
 Database cross references - Atp2b4
ExoCarta ExoCarta_29600
Entrez Gene 29600
UniProt Q64542  
 Atp2b4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Atp2b4
Molecular Function
    PDZ domain binding GO:0030165 IPI
    ATP binding GO:0005524 IEA
    scaffold protein binding GO:0097110 ISO
    metal ion binding GO:0046872 IEA
    calcium-transporting ATPase activity GO:0005388 ISO
    calmodulin binding GO:0005516 ISO
    nitric-oxide synthase inhibitor activity GO:0036487 ISO
    nitric-oxide synthase binding GO:0050998 ISO
    sodium channel regulator activity GO:0017080 ISO
    protein phosphatase 2B binding GO:0030346 ISO
Biological Process
    negative regulation of the force of heart contraction GO:0098736 ISO
    calcium ion export GO:1901660 IMP
    cellular response to epinephrine stimulus GO:0071872 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 ISO
    negative regulation of citrulline biosynthetic process GO:1903249 ISO
    neural retina development GO:0003407 IEP
    negative regulation of nitric oxide biosynthetic process GO:0045019 ISO
    positive regulation of peptidyl-serine phosphorylation GO:0033138 ISO
    response to hydrostatic pressure GO:0051599 ISO
    positive regulation of cAMP-dependent protein kinase activity GO:2000481 ISO
    negative regulation of adrenergic receptor signaling pathway involved in heart process GO:1901205 ISO
    negative regulation of nitric-oxide synthase activity GO:0051001 ISO
    calcium ion transport GO:0006816 IDA
    negative regulation of arginine catabolic process GO:1900082 ISO
    negative regulation of nitric oxide mediated signal transduction GO:0010751 ISO
    hippocampus development GO:0021766 IEP
    cellular calcium ion homeostasis GO:0006874 TAS
    calcium ion transmembrane transport GO:0070588 ISO
    negative regulation of calcineurin-NFAT signaling cascade GO:0070885 ISO
    spermatogenesis GO:0007283 IEP
    negative regulation of cardiac muscle hypertrophy in response to stress GO:1903243 ISO
    regulation of cell cycle G1/S phase transition GO:1902806 ISO
    metabolic process GO:0008152 IEA
Subcellular Localization
    sperm principal piece GO:0097228 IDA
    basolateral plasma membrane GO:0016323 IDA
    membrane GO:0016020 ISO
    Z disc GO:0030018 ISO
    integral component of membrane GO:0016021 IEA
    protein complex GO:0043234 ISO
    sarcolemma GO:0042383 ISO
    T-tubule GO:0030315 ISO
    plasma membrane GO:0005886 ISO
    neuron projection GO:0043005 IDA
 Experiment description of studies that identified Atp2b4 in exosomes
1
Experiment ID 90
ISEV standards
EM
EV Biophysical techniques
HSC70|HSP90|TSG101
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 94
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 99
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [QSTAR]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Atp2b4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Atp2b4 is involved
No pathways found





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