Gene description for Tm9sf4
Gene name transmembrane 9 superfamily protein member 4
Gene symbol Tm9sf4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Tm9sf4
ExoCarta ExoCarta_296279
Vesiclepedia VP_296279
Entrez Gene 296279
UniProt Q4KLL4  
 Tm9sf4 identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Tm9sf4
Biological Process
    response to hypoxia GO:0001666 IEA
    response to hypoxia GO:0001666 ISO
    phagocytosis GO:0006909 IEA
    phagocytosis GO:0006909 ISO
    cell adhesion GO:0007155 IEA
    cell adhesion GO:0007155 ISO
    intracellular monoatomic cation homeostasis GO:0030003 IEA
    regulation of intracellular pH GO:0051453 IEA
    regulation of intracellular pH GO:0051453 ISO
    vacuolar proton-transporting V-type ATPase complex assembly GO:0070072 IEA
    vacuolar proton-transporting V-type ATPase complex assembly GO:0070072 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 IEA
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISO
    positive regulation of protein exit from endoplasmic reticulum GO:0070863 ISS
    protein localization to membrane GO:0072657 IBA
    positive regulation of protein localization to cell surface GO:2000010 IEA
    positive regulation of protein localization to cell surface GO:2000010 ISO
    positive regulation of protein localization to cell surface GO:2000010 ISS
Subcellular Localization
    cytoplasm GO:0005737 IEA
    early endosome GO:0005769 IEA
    early endosome GO:0005769 ISO
    early endosome GO:0005769 ISS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    membrane GO:0016020 IBA
    membrane GO:0016020 IEA
    intracellular membrane-bounded organelle GO:0043231 IEA
 Experiment description of studies that identified Tm9sf4 in sEVs
1
Experiment ID 90
MISEV standards
✔
EM
Biophysical techniques
✔
HSC70|HSP90|TSG101|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 96
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 97
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Tm9sf4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Tm9sf4 is involved
No pathways found





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