Gene description for Phpt1
Gene name phosphohistidine phosphatase 1
Gene symbol Phpt1
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Phpt1
ExoCarta ExoCarta_296571
Entrez Gene 296571
 Phpt1 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Phpt1
Molecular Function
    ion channel binding GO:0044325 ISO
    calcium channel inhibitor activity GO:0019855 ISO
    phosphohistidine phosphatase activity GO:0008969 ISO
Biological Process
    protein dephosphorylation GO:0006470 ISO
    peptidyl-histidine dephosphorylation GO:0035971 ISO
    negative regulation of lyase activity GO:0051350 ISO
    negative regulation of ATP citrate synthase activity GO:2000984 ISO
    negative regulation of T cell receptor signaling pathway GO:0050860 ISO
    regulation of actin cytoskeleton reorganization GO:2000249 ISO
    positive regulation of cell motility GO:2000147 ISO
    positive regulation of insulin secretion involved in cellular response to glucose stimulus GO:0035774 IMP
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cytosol GO:0005829 ISO
 Experiment description of studies that identified Phpt1 in exosomes
1
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Phpt1
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Phpt1 is involved
No pathways found





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