Gene description for Psma4
Gene name proteasome (prosome, macropain) subunit, alpha type 4
Gene symbol Psma4
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Psma4
ExoCarta ExoCarta_29671
Vesiclepedia VP_29671
Entrez Gene 29671
UniProt P21670  
 Psma4 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Psma4
Molecular Function
    endopeptidase activity GO:0004175 IBA
    threonine-type endopeptidase activity GO:0004298 IEA
Biological Process
    proteasomal ubiquitin-independent protein catabolic process GO:0010499 IBA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    nucleus GO:0005634 ISO
    cytoplasm GO:0005737 ISO
    proteasome core complex GO:0005839 ISS
    proteasome complex GO:0000502 ISO
    intracellular membrane-bounded organelle GO:0043231 ISO
    extracellular exosome GO:0070062 ISO
    cytoplasmic mRNA processing body GO:0000932 ISS
    plasma membrane GO:0005886 ISO
    proteasome core complex, alpha-subunit complex GO:0019773 IEA
 Experiment description of studies that identified Psma4 in exosomes
1
Experiment ID 95
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 97
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70|MHCI
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Psma4
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Psma4 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells IEA Reactome
Antigen processing: Ubiquitination & Proteasome degradation IEA Reactome
APC/C:Cdc20 mediated degradation of Securin IEA Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 IEA Reactome
Asymmetric localization of PCP proteins IEA Reactome
AUF1 (hnRNP D0) destabilizes mRNA IEA Reactome
Autodegradation of Cdh1 by Cdh1:APC/C IEA Reactome
Autodegradation of the E3 ubiquitin ligase COP1 IEA Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDK-mediated phosphorylation and removal of Cdc6 IEA Reactome
CDT1 association with the CDC6:ORC:origin complex IEA Reactome
CLEC7A (Dectin-1) signaling IEA Reactome
Cross-presentation of soluble exogenous antigens (endosomes) IEA Reactome
Dectin-1 mediated noncanonical NF-kB signaling IEA Reactome
degradation of AXIN IEA Reactome
Degradation of beta-catenin by the destruction complex IEA Reactome
degradation of DVL IEA Reactome
Degradation of GLI1 by the proteasome IEA Reactome
Degradation of GLI2 by the proteasome IEA Reactome
ER-Phagosome pathway IEA Reactome
GLI3 is processed to GLI3R by the proteasome IEA Reactome
Hedgehog 'on' state IEA Reactome
Hedgehog ligand biogenesis IEA Reactome
Orc1 removal from chromatin IEA Reactome
Orc1 removal from chromatin IEA Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation IEA Reactome
Regulation of ornithine decarboxylase (ODC) IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF(Skp2)-mediated degradation of p27/p21 IEA Reactome
SCF-beta-TrCP mediated degradation of Emi1 IEA Reactome
Separation of Sister Chromatids IEA Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A IEA Reactome
Ubiquitin-dependent degradation of Cyclin D1 IEA Reactome





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