Gene description for Psma5
Gene name proteasome (prosome, macropain) subunit, alpha type 5
Gene symbol Psma5
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Psma5
ExoCarta ExoCarta_29672
Entrez Gene 29672
UniProt P34064  
 Psma5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Psma5
Molecular Function
    endopeptidase activity GO:0004175 IBA
    threonine-type endopeptidase activity GO:0004298 IEA
Biological Process
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 IEA
    proteasomal ubiquitin-independent protein catabolic process GO:0010499 IBA
Subcellular Localization
    proteasome complex GO:0000502 ISO
    extracellular exosome GO:0070062 ISO
    proteasome core complex GO:0005839 ISS
    nucleoplasm GO:0005654 ISO
    proteasome core complex, alpha-subunit complex GO:0019773 IEA
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 ISO
 Experiment description of studies that identified Psma5 in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Psma5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Psma5 is involved
No pathways found





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