Gene description for Napepld
Gene name N-acyl phosphatidylethanolamine phospholipase D
Gene symbol Napepld
Other names/aliases NAPE-PLD
Species Rattus norvegicus
 Database cross references - Napepld
ExoCarta ExoCarta_296757
Vesiclepedia VP_296757
Entrez Gene 296757
UniProt Q769K2  
 Napepld identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Napepld
Molecular Function
    phospholipase activity GO:0004620 IDA
    zinc ion binding GO:0008270 IEA
    zinc ion binding GO:0008270 ISO
    zinc ion binding GO:0008270 ISS
    bile acid binding GO:0032052 IEA
    bile acid binding GO:0032052 ISO
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 IBA
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 IEA
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 ISO
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 ISS
Biological Process
    temperature homeostasis GO:0001659 ISO
    temperature homeostasis GO:0001659 ISS
    phospholipid metabolic process GO:0006644 IDA
    phospholipid catabolic process GO:0009395 IEA
    response to isolation stress GO:0035900 IEP
    host-mediated regulation of intestinal microbiota composition GO:0048874 ISO
    host-mediated regulation of intestinal microbiota composition GO:0048874 ISS
    positive regulation of inflammatory response GO:0050729 ISO
    positive regulation of inflammatory response GO:0050729 ISS
    N-acylethanolamine metabolic process GO:0070291 IBA
    N-acylethanolamine metabolic process GO:0070291 ISO
    N-acylphosphatidylethanolamine metabolic process GO:0070292 IBA
    N-acylphosphatidylethanolamine metabolic process GO:0070292 IEA
    N-acylphosphatidylethanolamine metabolic process GO:0070292 ISO
    N-acylphosphatidylethanolamine metabolic process GO:0070292 ISS
    positive regulation of brown fat cell differentiation GO:0090336 ISO
    positive regulation of brown fat cell differentiation GO:0090336 ISS
    negative regulation of eating behavior GO:1903999 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    nuclear envelope GO:0005635 ISS
    nucleoplasm GO:0005654 ISS
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 ISS
    Golgi apparatus GO:0005794 IEA
    Golgi apparatus GO:0005794 ISO
    Golgi apparatus GO:0005794 ISS
    smooth endoplasmic reticulum membrane GO:0030868 ISO
    early endosome membrane GO:0031901 IEA
    presynaptic membrane GO:0042734 ISO
    membrane-bounded organelle GO:0043227 IBA
    postsynaptic membrane GO:0045211 ISO
    hippocampal mossy fiber to CA3 synapse GO:0098686 ISO
    presynapse GO:0098793 ISO
 Experiment description of studies that identified Napepld in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Napepld
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Napepld is involved
No pathways found





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