Gene description for Napepld
Gene name N-acyl phosphatidylethanolamine phospholipase D
Gene symbol Napepld
Other names/aliases NAPE-PLD
Species Rattus norvegicus
 Database cross references - Napepld
ExoCarta ExoCarta_296757
Entrez Gene 296757
UniProt Q769K2  
 Napepld identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Napepld
Molecular Function
    zinc ion binding GO:0008270 IEA
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 ISO
    phospholipase activity GO:0004620 IMP
Biological Process
    phospholipid catabolic process GO:0009395 IEA
    phospholipid metabolic process GO:0006644 TAS
Subcellular Localization
    membrane GO:0016020 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
 Experiment description of studies that identified Napepld in exosomes
1
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Napepld
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Napepld is involved
No pathways found





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