Gene description for Prkar2a
Gene name protein kinase, cAMP dependent regulatory, type II alpha
Gene symbol Prkar2a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prkar2a
ExoCarta ExoCarta_29699
Entrez Gene 29699
UniProt P12368  
 Prkar2a identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Prkar2a
Molecular Function
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    small molecule binding GO:0036094 IDA
    kinase activity GO:0016301 IEA
    cAMP binding GO:0030552 IDA
    protein domain specific binding GO:0019904 IPI
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    ubiquitin protein ligase binding GO:0031625 ISO
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein kinase A catalytic subunit binding GO:0034236 ISO
Biological Process
    response to clozapine GO:0097338 IEP
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 ISO
    phosphorylation GO:0016310 IEA
    protein heterooligomerization GO:0051291 IDA
    regulation of protein kinase activity GO:0045859 IDA
Subcellular Localization
    extracellular exosome GO:0070062 ISO
    cAMP-dependent protein kinase complex GO:0005952 IEA
    membrane GO:0016020 ISO
    cytoplasm GO:0005737 ISO
    focal adhesion GO:0005925 ISO
    T-tubule GO:0030315 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    AMP-activated protein kinase complex GO:0031588 ISO
    protein complex GO:0043234 IDA
    plasma membrane GO:0005886 ISO
    centrosome GO:0005813 ISO
 Experiment description of studies that identified Prkar2a in exosomes
1
Experiment ID 225
ISEV standards
✔
EM
EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✔
CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors Lee JE, Moon PG, Lee IK, Baek MC
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP90|HSC70
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 192
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
CD63|CD81|FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prkar2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkar2a is involved
PathwayEvidenceSource
Glucagon-like Peptide-1 (GLP1) regulates insulin secretion IEA Reactome





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