Gene description for Prkar2a
Gene name protein kinase, cAMP dependent regulatory, type II alpha
Gene symbol Prkar2a
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Prkar2a
ExoCarta ExoCarta_29699
Vesiclepedia VP_29699
Entrez Gene 29699
UniProt P12368  
 Prkar2a identified in exosomes derived from the following tissue/cell type
Adipocytes 25998041    
Reticulocytes 21828046    
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Prkar2a
Molecular Function
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IBA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 IEA
    cAMP-dependent protein kinase inhibitor activity GO:0004862 ISO
    protein binding GO:0005515 IPI
    cAMP-dependent protein kinase regulator activity GO:0008603 IDA
    cAMP-dependent protein kinase regulator activity GO:0008603 ISO
    protein domain specific binding GO:0019904 IDA
    protein domain specific binding GO:0019904 IEA
    protein domain specific binding GO:0019904 IPI
    protein domain specific binding GO:0019904 ISO
    cAMP binding GO:0030552 IBA
    cAMP binding GO:0030552 IDA
    cAMP binding GO:0030552 IEA
    ubiquitin protein ligase binding GO:0031625 IEA
    ubiquitin protein ligase binding GO:0031625 ISO
    beta-2 adrenergic receptor binding GO:0031698 IPI
    protein kinase A catalytic subunit binding GO:0034236 IBA
    protein kinase A catalytic subunit binding GO:0034236 IC
    protein kinase A catalytic subunit binding GO:0034236 IEA
    protein kinase A catalytic subunit binding GO:0034236 ISO
    small molecule binding GO:0036094 IDA
    identical protein binding GO:0042802 IPI
    protein-containing complex binding GO:0044877 IDA
Biological Process
    adenylate cyclase-activating G protein-coupled receptor signaling pathway GO:0007189 IBA
    regulation of protein kinase activity GO:0045859 IDA
    modulation of chemical synaptic transmission GO:0050804 ISO
    negative regulation of cAMP/PKA signal transduction GO:0141162 IEA
    negative regulation of cAMP/PKA signal transduction GO:0141162 ISO
    regulation of meiotic cell cycle process involved in oocyte maturation GO:1903538 ISO
    positive regulation of meiotic cell cycle process involved in oocyte maturation GO:1904146 ISO
    negative regulation of cAMP-dependent protein kinase activity GO:2000480 ISO
Subcellular Localization
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    centrosome GO:0005813 IEA
    centrosome GO:0005813 ISO
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    axoneme GO:0005930 IEA
    axoneme GO:0005930 ISO
    cAMP-dependent protein kinase complex GO:0005952 IBA
    cAMP-dependent protein kinase complex GO:0005952 IDA
    cAMP-dependent protein kinase complex GO:0005952 IEA
    cAMP-dependent protein kinase complex GO:0005952 ISO
    T-tubule GO:0030315 IDA
    nucleotide-activated protein kinase complex GO:0031588 IEA
    nucleotide-activated protein kinase complex GO:0031588 ISO
    protein-containing complex GO:0032991 IDA
    protein-containing complex GO:0032991 ISO
    germinal vesicle GO:0042585 ISO
    plasma membrane raft GO:0044853 IEA
    plasma membrane raft GO:0044853 ISO
    synapse GO:0045202 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    glutamatergic synapse GO:0098978 ISO
 Experiment description of studies that identified Prkar2a in exosomes
1
Experiment ID 225
MISEV standards
EM
Biophysical techniques
GAPDH|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25998041    
Organism Rattus norvegicus
Experiment description Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats.
Authors "Lee JE, Moon PG, Lee IK, Baek MC"
Journal name Protein J
Publication year 2015
Sample Adipocytes
Sample name Adipocytes
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
3
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
4
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Prkar2a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Prkar2a is involved
PathwayEvidenceSource
Activation of NMDA receptors and postsynaptic events IEA Reactome
Aquaporin-mediated transport IEA Reactome
Ca-dependent events IEA Reactome
Calmodulin induced events IEA Reactome
CaM pathway IEA Reactome
Cellular responses to mechanical stimuli IEA Reactome
Cellular responses to stimuli IEA Reactome
CREB1 phosphorylation through the activation of Adenylate Cyclase IEA Reactome
DAG and IP3 signaling IEA Reactome
DARPP-32 events IEA Reactome
Factors involved in megakaryocyte development and platelet production IEA Reactome
G alpha (i) signalling events IEA Reactome
G alpha (s) signalling events IEA Reactome
G-protein mediated events IEA Reactome
Glucagon signaling in metabolic regulation IEA Reactome
GPCR downstream signalling IEA Reactome
GPER1 signaling IEA Reactome
Hedgehog 'off' state IEA Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells IEA Reactome
Integration of energy metabolism IEA Reactome
Intracellular signaling by second messengers IEA Reactome
Metabolism IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Opioid Signalling IEA Reactome
PKA activation IEA Reactome
PKA activation in glucagon signalling IEA Reactome
PKA-mediated phosphorylation of CREB IEA Reactome
PLC beta mediated events IEA Reactome
Post NMDA receptor activation events IEA Reactome
Response of endothelial cells to shear stress IEA Reactome
Signal Transduction IEA Reactome
Signaling by GPCR IEA Reactome
Signaling by Hedgehog IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of small molecules IEA Reactome
Vasopressin regulates renal water homeostasis via Aquaporins IEA Reactome





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