Experiment description of studies that identified Prkar2a in exosomes |
1 |
Experiment ID |
225 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
GAPDH|CD63
|
Enriched markers |
✘
|
Negative markers |
✔
NTA
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25998041
|
Organism |
Rattus norvegicus |
Experiment description |
Proteomic Analysis of Extracellular Vesicles Released by Adipocytes of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats. |
Authors |
"Lee JE, Moon PG, Lee IK, Baek MC" |
Journal name |
Protein J
|
Publication year |
2015 |
Sample |
Adipocytes |
Sample name |
Adipocytes |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
2 |
Experiment ID |
95 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 1 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
3 |
Experiment ID |
98 |
MISEV standards |
✔
EM
|
Biophysical techniques |
✔
TSG101|HSP90|HSC70|MHCI
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [Orbitrap]
|
PubMed ID |
21828046
|
Organism |
Rattus norvegicus |
Experiment description |
The proteo-lipidic composition of exosomes changes during reticulocyte maturation. |
Authors |
"Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E." |
Journal name |
JBC
|
Publication year |
2011 |
Sample |
Reticulocytes |
Sample name |
Reticulocytes - D7 Rep 2 |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation Sucrose density gradient |
Flotation density |
1.13-1.25 g/mL
|
Molecules identified in the study |
Protein Lipids |
Methods used in the study |
Mass spectrometry [QSTAR] Mass spectrometry [Orbitrap] Western blotting |
|
|
4 |
Experiment ID |
192 |
MISEV standards |
✔
CEM
|
Biophysical techniques |
✔
TSG101|HSP70|CD63|CD81|FLOT
|
Enriched markers |
✘
|
Negative markers |
✘
|
Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20535238
|
Organism |
Rattus norvegicus |
Experiment description |
Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples. |
Authors |
"Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M." |
Journal name |
Proteomics Clin Appl
|
Publication year |
2010 |
Sample |
Urine |
Sample name |
Urine - Rats of liver injury |
Isolation/purification methods |
Differential centrifugation Filtration UltracentrifugationSucrose cushion Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|