Gene description for Nagk
Gene name N-acetylglucosamine kinase
Gene symbol Nagk
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Nagk
ExoCarta ExoCarta_297393
Vesiclepedia VP_297393
Entrez Gene 297393
UniProt P81799  
 Nagk identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Nagk
Molecular Function
    ATP binding GO:0005524 IEA
    N-acylmannosamine kinase activity GO:0009384 ISO
    N-acetylglucosamine kinase activity GO:0045127 IBA
    N-acetylglucosamine kinase activity GO:0045127 IEA
    N-acetylglucosamine kinase activity GO:0045127 ISO
    N-acetylglucosamine kinase activity GO:0045127 ISS
    muramyl dipeptide kinase activity GO:0160047 ISO
    muramyl dipeptide kinase activity GO:0160047 ISS
Biological Process
    N-acetylglucosamine metabolic process GO:0006044 ISO
    N-acetylglucosamine catabolic process GO:0006046 ISO
    UDP-N-acetylglucosamine biosynthetic process GO:0006048 ISO
    N-acetylneuraminate catabolic process GO:0019262 IEA
    response to muramyl dipeptide GO:0032495 ISO
    response to muramyl dipeptide GO:0032495 ISS
    defense response to bacterium GO:0042742 ISO
    defense response to bacterium GO:0042742 ISS
    innate immune response GO:0045087 IEA
    carbohydrate phosphorylation GO:0046835 IEA
    positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070434 ISO
    positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway GO:0070434 ISS
 Experiment description of studies that identified Nagk in sEVs
1
Experiment ID 96
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nagk
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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