Gene description for Nampt
Gene name nicotinamide phosphoribosyltransferase
Gene symbol Nampt
Other names/aliases Pbef
Pbef1
Species Rattus norvegicus
 Database cross references - Nampt
ExoCarta ExoCarta_297508
Entrez Gene 297508
UniProt Q80Z29  
 Nampt identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Nampt
Molecular Function
    drug binding GO:0008144 IDA
    cytokine activity GO:0005125 IDA
    nicotinamide phosphoribosyltransferase activity GO:0047280 ISO
    protein homodimerization activity GO:0042803 IDA
    nicotinate-nucleotide diphosphorylase (carboxylating) activity GO:0004514 IEA
Biological Process
    female pregnancy GO:0007565 IEP
    adipose tissue development GO:0060612 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 IMP
    NAD biosynthetic process GO:0009435 ISO
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
    response to organic cyclic compound GO:0014070 IEP
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 ISO
    circadian regulation of gene expression GO:0032922 ISS
    insulin receptor signaling pathway GO:0008286 ISO
Subcellular Localization
    extracellular space GO:0005615 IEA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    nucleus GO:0005634 IDA
 Experiment description of studies that identified Nampt in exosomes
1
Experiment ID 95
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nampt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Nampt is involved
PathwayEvidenceSource
Nicotinamide salvaging IEA Reactome





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