Gene description for Nampt
Gene name nicotinamide phosphoribosyltransferase
Gene symbol Nampt
Other names/aliases Pbef
Pbef1
Species Rattus norvegicus
 Database cross references - Nampt
ExoCarta ExoCarta_297508
Vesiclepedia VP_297508
Entrez Gene 297508
UniProt Q80Z29  
 Nampt identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Nampt
Molecular Function
    nucleotide binding GO:0000166 IDA
    cytokine activity GO:0005125 IDA
    cytokine activity GO:0005125 IEA
    identical protein binding GO:0042802 IDA
    identical protein binding GO:0042802 IEA
    identical protein binding GO:0042802 ISO
    nicotinamide phosphoribosyltransferase activity GO:0047280 IBA
    nicotinamide phosphoribosyltransferase activity GO:0047280 IDA
    nicotinamide phosphoribosyltransferase activity GO:0047280 IEA
    nicotinamide phosphoribosyltransferase activity GO:0047280 ISO
    heterocyclic compound binding GO:1901363 IDA
Biological Process
    microglial cell activation GO:0001774 IMP
    signal transduction GO:0007165 IEA
    female pregnancy GO:0007565 IEP
    circadian rhythm GO:0007623 IEP
    circadian rhythm GO:0007623 ISO
    insulin receptor signaling pathway GO:0008286 ISO
    NAD biosynthetic process GO:0009435 IBA
    NAD biosynthetic process GO:0009435 IEA
    NAD biosynthetic process GO:0009435 ISO
    negative regulation of autophagy GO:0010507 IMP
    response to organic cyclic compound GO:0014070 IEP
    regulation of lung blood pressure GO:0014916 IMP
    circadian regulation of gene expression GO:0032922 IEA
    circadian regulation of gene expression GO:0032922 ISO
    circadian regulation of gene expression GO:0032922 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 ISO
    positive regulation of smooth muscle cell proliferation GO:0048661 IMP
    adipose tissue development GO:0060612 ISO
    cellular response to hypoxia GO:0071456 IEP
    cellular response to ionizing radiation GO:0071479 IEP
    cellular response to amyloid-beta GO:1904646 IEP
    response to D-galactose GO:1905377 IEP
    negative regulation of cellular senescence GO:2000773 IDA
Subcellular Localization
    extracellular space GO:0005615 IEA
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IEA
    cytoplasm GO:0005737 ISO
    nuclear speck GO:0016607 IEA
    nuclear speck GO:0016607 ISO
    cell junction GO:0030054 IEA
    cell junction GO:0030054 ISO
 Experiment description of studies that identified Nampt in sEVs
1
Experiment ID 95
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Nampt
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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