Gene description for Dppa3
Gene name developmental pluripotency-associated 3
Gene symbol Dppa3
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dppa3
ExoCarta ExoCarta_297576
Vesiclepedia VP_297576
Entrez Gene 297576
UniProt Q6IMK0  
 Dppa3 identified in exosomes derived from the following tissue/cell type
Pancreatic cancer cells 20124479    
Pancreatic cancer cells 20124479    
 Gene ontology annotations for Dppa3
Molecular Function
    methylated histone binding GO:0035064 ISS
Biological Process
    chromatin modification GO:0016568 IEA
    negative regulation of DNA demethylation GO:1901536 ISS
    regulation of genetic imprinting GO:2000653 ISS
    protection of DNA demethylation of female pronucleus GO:0044726 ISS
Subcellular Localization
    female pronucleus GO:0001939 ISS
    male pronucleus GO:0001940 ISS
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified Dppa3 in exosomes
1
Experiment ID 92
MISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH|CD9|CD151
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
RatRef-12 Microarray
FACS
Mass spectrometry [MALDI TOF]
2
Experiment ID 93
MISEV standards
EM
EV Biophysical techniques
HSP70|GAPDH|CD9|CD151
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 20124479    
Organism Rattus norvegicus
Experiment description Cell surface tetraspanin Tspan8 contributes to molecular pathways of exosome-induced endothelial cell activation.
Authors Nazarenko I, Rana S, Baumann A, McAlear J, Hellwig A, Trendelenburg M, Lochnit G, Preissner KT, Zoller M.
Journal name CR
Publication year 2010
Sample Pancreatic cancer cells
Sample name AS-Tspan8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.14-1.17 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study RT-PCR
Western blotting
 Protein-protein interactions for Dppa3
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dppa3 is involved
No pathways found





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