Gene description for Chmp5
Gene name charged multivesicular body protein 5
Gene symbol Chmp5
Other names/aliases RGD1305968
Species Rattus norvegicus
 Database cross references - Chmp5
ExoCarta ExoCarta_297995
Entrez Gene 297995
UniProt Q4QQV8  
 Chmp5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Chmp5
Biological Process
    viral budding GO:0046755 ISO
    regulation of centrosome duplication GO:0010824 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    lysosome organization GO:0007040 ISO
    nucleus organization GO:0006997 ISO
    mitotic metaphase plate congression GO:0007080 ISO
    vacuolar transport GO:0007034 IEA
    regulation of receptor recycling GO:0001919 ISO
    protein transport GO:0015031 IEA
    cell separation after cytokinesis GO:0000920 ISO
    multivesicular body sorting pathway GO:0071985 ISO
    endosome to lysosome transport GO:0008333 ISO
Subcellular Localization
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    endosome membrane GO:0010008 IEA
    nucleus GO:0005634 ISO
    intracellular GO:0005622 ISO
    cell GO:0005623 ISO
 Experiment description of studies that identified Chmp5 in exosomes
1
Experiment ID 90
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70|HSP90|TSG101
EV Cytosolic markers
✔
MHCI
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 192
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
CD63|CD81|FLOT
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M.
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chmp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Chmp5 is involved
PathwayEvidenceSource
Endosomal Sorting Complex Required For Transport (ESCRT) IEA Reactome





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