Gene description for Chmp5
Gene name charged multivesicular body protein 5
Gene symbol Chmp5
Other names/aliases RGD1305968
Species Rattus norvegicus
 Database cross references - Chmp5
ExoCarta ExoCarta_297995
Vesiclepedia VP_297995
Entrez Gene 297995
UniProt Q4QQV8  
 Chmp5 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Urine 20535238    
 Gene ontology annotations for Chmp5
Biological Process
    plasma membrane repair GO:0001778 ISO
    regulation of receptor recycling GO:0001919 ISO
    vesicle budding from membrane GO:0006900 IBA
    autophagy GO:0006914 ISO
    nucleus organization GO:0006997 ISO
    lysosome organization GO:0007040 ISO
    mitotic metaphase chromosome alignment GO:0007080 ISO
    endosome to lysosome transport GO:0008333 ISO
    regulation of centrosome duplication GO:0010824 ISO
    protein transport GO:0015031 IEA
    erythrocyte differentiation GO:0030218 ISO
    nuclear membrane reassembly GO:0031468 ISO
    late endosome to vacuole transport via multivesicular body sorting pathway GO:0032511 IBA
    viral budding via host ESCRT complex GO:0039702 ISO
    ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway GO:0043162 ISO
    viral budding from plasma membrane GO:0046761 ISO
    midbody abscission GO:0061952 ISO
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to lipopolysaccharide GO:0071222 ISS
    cellular response to muramyl dipeptide GO:0071225 ISO
    cellular response to muramyl dipeptide GO:0071225 ISS
    multivesicular body sorting pathway GO:0071985 ISO
    autophagosome maturation GO:0097352 ISO
    regulation of mitotic spindle assembly GO:1901673 ISO
    late endosome to lysosome transport GO:1902774 ISO
Subcellular Localization
    autophagosome membrane GO:0000421 ISO
    kinetochore GO:0000776 ISO
    nucleus GO:0005634 ISO
    nuclear pore GO:0005643 ISO
    lysosomal membrane GO:0005765 ISO
    multivesicular body GO:0005771 IBA
    kinetochore microtubule GO:0005828 ISO
    cytosol GO:0005829 ISO
    plasma membrane GO:0005886 ISO
    midbody GO:0030496 ISO
    multivesicular body membrane GO:0032585 ISO
    amphisome membrane GO:1904930 ISO
 Experiment description of studies that identified Chmp5 in exosomes
1
Experiment ID 90
MISEV standards
EM
Biophysical techniques
HSC70|HSP90|TSG101|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E"
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 192
MISEV standards
CEM
Biophysical techniques
TSG101|HSP70|CD63|CD81|FLOT
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20535238    
Organism Rattus norvegicus
Experiment description Candidate biomarkers in exosome-like vesicles purified from rat and mouse urine samples.
Authors "Conde-Vancells J, Rodriguez-Suarez E, Gonzalez E, Berisa A, Gil D, Embade N, Valle M, Luka Z, Elortza F, Wagner C, Lu SC, Mato JM, Falcon-Perez M."
Journal name Proteomics Clin Appl
Publication year 2010
Sample Urine
Sample name Urine - Rats of liver injury
Isolation/purification methods Differential centrifugation
Filtration
UltracentrifugationSucrose cushion
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Chmp5
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here