Gene description for Sdhb
Gene name succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene symbol Sdhb
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Sdhb
ExoCarta ExoCarta_298596
Vesiclepedia VP_298596
Entrez Gene 298596
UniProt P21913  
 Sdhb identified in sEVs derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Sdhb
Molecular Function
    succinate dehydrogenase (quinone) activity GO:0008177 IEA
    succinate dehydrogenase (quinone) activity GO:0008177 IMP
    succinate dehydrogenase (quinone) activity GO:0008177 ISO
    succinate dehydrogenase (quinone) activity GO:0008177 ISS
    electron transfer activity GO:0009055 IEA
    oxidoreductase activity GO:0016491 IEA
    metal ion binding GO:0046872 IEA
    ubiquinone binding GO:0048039 ISO
    ubiquinone binding GO:0048039 ISS
    2 iron, 2 sulfur cluster binding GO:0051537 IEA
    2 iron, 2 sulfur cluster binding GO:0051537 ISO
    2 iron, 2 sulfur cluster binding GO:0051537 ISS
    3 iron, 4 sulfur cluster binding GO:0051538 IEA
    3 iron, 4 sulfur cluster binding GO:0051538 ISO
    3 iron, 4 sulfur cluster binding GO:0051538 ISS
    4 iron, 4 sulfur cluster binding GO:0051539 IEA
    4 iron, 4 sulfur cluster binding GO:0051539 ISO
    4 iron, 4 sulfur cluster binding GO:0051539 ISS
Biological Process
    tricarboxylic acid cycle GO:0006099 IEA
    tricarboxylic acid cycle GO:0006099 NAS
    succinate metabolic process GO:0006105 IMP
    mitochondrial electron transport, succinate to ubiquinone GO:0006121 NAS
    aerobic respiration GO:0009060 IBA
    respiratory electron transport chain GO:0022904 IBA
    respiratory electron transport chain GO:0022904 IMP
    proton motive force-driven mitochondrial ATP synthesis GO:0042776 NAS
Subcellular Localization
    nucleoplasm GO:0005654 IEA
    nucleoplasm GO:0005654 ISO
    mitochondrion GO:0005739 ISO
    mitochondrial inner membrane GO:0005743 IDA
    mitochondrial inner membrane GO:0005743 IEA
    mitochondrial inner membrane GO:0005743 ISO
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial inner membrane GO:0005743 NAS
    plasma membrane GO:0005886 IEA
    plasma membrane GO:0005886 ISO
    mitochondrial membrane GO:0031966 IBA
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 IEA
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 ISO
    respiratory chain complex II (succinate dehydrogenase) GO:0045273 ISS
 Experiment description of studies that identified Sdhb in sEVs
1
Experiment ID 97
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
MISEV standards
EM
Biophysical techniques
TSG101|HSP90|HSC70|MHCI
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors "Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E."
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Sdhb
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Dao  
Affinity Capture-MS Rattus norvegicus
2 Itm2b 290364
Affinity Capture-MS Rattus norvegicus
View the network image/svg+xml
 Pathways in which Sdhb is involved
PathwayEvidenceSource
Aerobic respiration and respiratory electron transport IEA Reactome
Citric acid cycle (TCA cycle) IEA Reactome
Metabolism IEA Reactome





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