Gene description for Med6
Gene name mediator complex subunit 6
Gene symbol Med6
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Med6
ExoCarta ExoCarta_299180
Entrez Gene 299180
 Med6 identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
 Gene ontology annotations for Med6
Molecular Function
    RNA polymerase II transcription cofactor activity GO:0001104 IEA
    ubiquitin protein ligase activity GO:0061630 IDA
    transcription coactivator activity GO:0003713 ISO
    transcription factor binding GO:0008134 ISO
    DNA binding GO:0003677 ISO
Biological Process
    protein ubiquitination GO:0016567 IDA
    stem cell maintenance GO:0019827 ISO
    regulation of transcription from RNA polymerase II promoter GO:0006357 IEA
    transcription from RNA polymerase II promoter GO:0006366 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    membrane GO:0016020 ISO
    nucleoplasm GO:0005654 ISO
    mediator complex GO:0016592 ISO
    ubiquitin ligase complex GO:0000151 IDA
 Experiment description of studies that identified Med6 in exosomes
1
Experiment ID 96
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D2 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Med6
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Med6 is involved
No pathways found





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