Gene description for Dlst
Gene name dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex)
Gene symbol Dlst
Other names/aliases -
Species Rattus norvegicus
 Database cross references - Dlst
ExoCarta ExoCarta_299201
Entrez Gene 299201
UniProt Q01205  
 Dlst identified in exosomes derived from the following tissue/cell type
Reticulocytes 21828046    
Reticulocytes 21828046    
 Gene ontology annotations for Dlst
Molecular Function
    heat shock protein binding GO:0031072 IPI
    dihydrolipoyllysine-residue succinyltransferase activity GO:0004149 IDA
    chaperone binding GO:0051087 IPI
Biological Process
    NADH metabolic process GO:0006734 IDA
    2-oxoglutarate metabolic process GO:0006103 IDA
    L-lysine catabolic process to acetyl-CoA via saccharopine GO:0033512 IEA
    tricarboxylic acid cycle GO:0006099 IC
Subcellular Localization
    membrane GO:0016020 ISO
    mitochondrion GO:0005739 ISO
    oxoglutarate dehydrogenase complex GO:0045252 IDA
    myelin sheath GO:0043209 ISO
    intracellular membrane-bounded organelle GO:0043231 IDA
    nucleus GO:0005634 ISO
    plasma membrane GO:0005886 IDA
    extracellular exosome GO:0070062 ISO
 Experiment description of studies that identified Dlst in exosomes
1
Experiment ID 97
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D4 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.16-1.21 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 98
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP90|HSC70
EV Cytosolic markers
MHCI
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [Orbitrap]
PubMed ID 21828046    
Organism Rattus norvegicus
Experiment description The proteo-lipidic composition of exosomes changes during reticulocyte maturation.
Authors Carayon K, Chaoui K, Ronzier E, Lazar I, Bertrand-Michel J, Roques V, Balor S, Terce F, Lopez A, Salome L, Joly E.
Journal name JBC
Publication year 2011
Sample Reticulocytes
Sample name Reticulocytes - D7 Rep 2
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.25 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Mass spectrometry [QSTAR]
Mass spectrometry [Orbitrap]
Western blotting
 Protein-protein interactions for Dlst
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Dlst is involved
No pathways found





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